guest ::
| Home > Publications database > Formation of Volutin Granules in Corynebacterium glutamicum |
| Home > Publications database > Formation of Volutin Granules in Corynebacterium glutamicum |
| Journal Article | PreJuSER-41953 |
; ; ; ; ; ;
2005
Wiley-Blackwell
Oxford [u.a.]
This record in other databases: 
Please use a persistent id in citations: doi:10.1016/j.femsle.2004.11.047
Abstract: Volutin granules are intracellular storages of complexed inorganic polyphosphate (poly P). Histochemical staining procedures differentiate between pathogenic corynebacteria such as Corynebacterum diphtheriae (containing volutin) and non-pathogenic species, such as C. glutamicum. Here we report that strains ATCC13032 and MH20-22B of the non-pathogenic C. glutamicum also formed subcellular entities (18-37% of the total cell volume) that had the typical characteristics of volutin granules: (i) volutin staining, (ii) green UV fluorescence when stained with 4',6-diamidino-2-phenylindole, (iii) electron-dense and rich in phosphorus when determined with transmission electron microscopy and X-ray microanalysis, and (iv) 31P NMR poly P resonances of isolated granules dissolved in EDTA. MgCl2 addition to the growth medium stimulated granule formation but did not effect expression of genes involved in poly P metabolism. Granular volutin fractions from lysed cells contained polyphosphate glucokinase as detected by SDS-PAGE/MALDI-TOF, indicating that this poly P metabolizing enzyme is present also in intact poly P granules. The results suggest that formation of volutin is a more widespread phenomenon than generally accepted.
Keyword(s): Bacterial Proteins: genetics (MeSH) ; Bacterial Proteins: metabolism (MeSH) ; Corynebacterium glutamicum: genetics (MeSH) ; Corynebacterium glutamicum: metabolism (MeSH) ; Corynebacterium glutamicum: ultrastructure (MeSH) ; Cytoplasmic Granules: metabolism (MeSH) ; Cytoplasmic Granules: ultrastructure (MeSH) ; Electron Probe Microanalysis (MeSH) ; Gene Expression Profiling (MeSH) ; Gene Expression Regulation, Bacterial (MeSH) ; Inorganic Chemicals: metabolism (MeSH) ; Magnesium Chloride: pharmacology (MeSH) ; Magnetic Resonance Spectroscopy (MeSH) ; Microscopy, Electron, Transmission (MeSH) ; Oligonucleotide Array Sequence Analysis (MeSH) ; Phosphorus Isotopes (MeSH) ; Phosphotransferases: metabolism (MeSH) ; Polyphosphates: metabolism (MeSH) ; Proteins: metabolism (MeSH) ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: methods (MeSH) ; Bacterial Proteins ; Inorganic Chemicals ; Phosphorus Isotopes ; Polyphosphates ; Proteins ; Magnesium Chloride ; Phosphotransferases ; polyphosphate-glucose phosphotransferase ; J ; Corynebacterium glutamicum (auto) ; inorganic polyphosphate (auto) ; volutin granules (auto) ; electron dispersive X-ray analysis (auto) ; DAPI fluorescence (auto) ; P-31 NMR (auto) ; magnesium (auto) ; polyphosphate glucokinase (auto) ; MALDI-TOF (auto) ; DNA microarray (auto)
|
The record appears in these collections: |