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000041953 0247_ $$2DOI$$a10.1016/j.femsle.2004.11.047
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000041953 084__ $$2WoS$$aMicrobiology
000041953 1001_ $$0P:(DE-Juel1)VDB42664$$aPallerla, S. R.$$b0$$uFZJ
000041953 245__ $$aFormation of Volutin Granules in Corynebacterium glutamicum
000041953 260__ $$aOxford [u.a.]$$bWiley-Blackwell$$c2005
000041953 300__ $$a133 - 140
000041953 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000041953 440_0 $$02056$$aFems Microbiology Letters$$v243$$x0378-1097$$y1
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000041953 520__ $$aVolutin granules are intracellular storages of complexed inorganic polyphosphate (poly P). Histochemical staining procedures differentiate between pathogenic corynebacteria such as Corynebacterum diphtheriae (containing volutin) and non-pathogenic species, such as C. glutamicum. Here we report that strains ATCC13032 and MH20-22B of the non-pathogenic C. glutamicum also formed subcellular entities (18-37% of the total cell volume) that had the typical characteristics of volutin granules: (i) volutin staining, (ii) green UV fluorescence when stained with 4',6-diamidino-2-phenylindole, (iii) electron-dense and rich in phosphorus when determined with transmission electron microscopy and X-ray microanalysis, and (iv) 31P NMR poly P resonances of isolated granules dissolved in EDTA. MgCl2 addition to the growth medium stimulated granule formation but did not effect expression of genes involved in poly P metabolism. Granular volutin fractions from lysed cells contained polyphosphate glucokinase as detected by SDS-PAGE/MALDI-TOF, indicating that this poly P metabolizing enzyme is present also in intact poly P granules. The results suggest that formation of volutin is a more widespread phenomenon than generally accepted.
000041953 536__ $$0G:(DE-Juel1)FUEK256$$2G:(DE-HGF)$$aBiotechnologie$$cL02$$x0
000041953 536__ $$0G:(DE-Juel1)FUEK257$$aChemie und Dynamik der Geo-Biosphäre$$cU01$$x1
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000041953 650_2 $$2MeSH$$aBacterial Proteins: genetics
000041953 650_2 $$2MeSH$$aBacterial Proteins: metabolism
000041953 650_2 $$2MeSH$$aCorynebacterium glutamicum: genetics
000041953 650_2 $$2MeSH$$aCorynebacterium glutamicum: metabolism
000041953 650_2 $$2MeSH$$aCorynebacterium glutamicum: ultrastructure
000041953 650_2 $$2MeSH$$aCytoplasmic Granules: metabolism
000041953 650_2 $$2MeSH$$aCytoplasmic Granules: ultrastructure
000041953 650_2 $$2MeSH$$aElectron Probe Microanalysis
000041953 650_2 $$2MeSH$$aGene Expression Profiling
000041953 650_2 $$2MeSH$$aGene Expression Regulation, Bacterial
000041953 650_2 $$2MeSH$$aInorganic Chemicals: metabolism
000041953 650_2 $$2MeSH$$aMagnesium Chloride: pharmacology
000041953 650_2 $$2MeSH$$aMagnetic Resonance Spectroscopy
000041953 650_2 $$2MeSH$$aMicroscopy, Electron, Transmission
000041953 650_2 $$2MeSH$$aOligonucleotide Array Sequence Analysis
000041953 650_2 $$2MeSH$$aPhosphorus Isotopes
000041953 650_2 $$2MeSH$$aPhosphotransferases: metabolism
000041953 650_2 $$2MeSH$$aPolyphosphates: metabolism
000041953 650_2 $$2MeSH$$aProteins: metabolism
000041953 650_2 $$2MeSH$$aSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: methods
000041953 650_7 $$00$$2NLM Chemicals$$aBacterial Proteins
000041953 650_7 $$00$$2NLM Chemicals$$aInorganic Chemicals
000041953 650_7 $$00$$2NLM Chemicals$$aPhosphorus Isotopes
000041953 650_7 $$00$$2NLM Chemicals$$aPolyphosphates
000041953 650_7 $$00$$2NLM Chemicals$$aProteins
000041953 650_7 $$07786-30-3$$2NLM Chemicals$$aMagnesium Chloride
000041953 650_7 $$0EC 2.7.-$$2NLM Chemicals$$aPhosphotransferases
000041953 650_7 $$0EC 2.7.1.63$$2NLM Chemicals$$apolyphosphate-glucose phosphotransferase
000041953 650_7 $$2WoSType$$aJ
000041953 65320 $$2Author$$aCorynebacterium glutamicum
000041953 65320 $$2Author$$ainorganic polyphosphate
000041953 65320 $$2Author$$avolutin granules
000041953 65320 $$2Author$$aelectron dispersive X-ray analysis
000041953 65320 $$2Author$$aDAPI fluorescence
000041953 65320 $$2Author$$aP-31 NMR
000041953 65320 $$2Author$$amagnesium
000041953 65320 $$2Author$$apolyphosphate glucokinase
000041953 65320 $$2Author$$aMALDI-TOF
000041953 65320 $$2Author$$aDNA microarray
000041953 7001_ $$0P:(DE-Juel1)VDB43392$$aKnebel, S.$$b1$$uFZJ
000041953 7001_ $$0P:(DE-Juel1)128982$$aPolen, T.$$b2$$uFZJ
000041953 7001_ $$0P:(DE-HGF)0$$aKlauth, P.$$b3
000041953 7001_ $$0P:(DE-HGF)0$$aHollender, J.$$b4
000041953 7001_ $$0P:(DE-Juel1)VDB1764$$aWendisch, V. F.$$b5$$uFZJ
000041953 7001_ $$0P:(DE-Juel1)VDB10831$$aSchoberth, S. M.$$b6$$uFZJ
000041953 773__ $$0PERI:(DE-600)1501716-3$$a10.1016/j.femsle.2004.11.047$$gVol. 243, p. 133 - 140$$p133 - 140$$q243<133 - 140$$tFEMS microbiology letters$$v243$$x0378-1097$$y2005
000041953 8567_ $$uhttp://dx.doi.org/10.1016/j.femsle.2004.11.047
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