TY  - JOUR
AU  - Pallerla, S. R.
AU  - Knebel, S.
AU  - Polen, T.
AU  - Klauth, P.
AU  - Hollender, J.
AU  - Wendisch, V. F.
AU  - Schoberth, S. M.
TI  - Formation of Volutin Granules in Corynebacterium glutamicum
JO  - FEMS microbiology letters
VL  - 243
SN  - 0378-1097
CY  - Oxford [u.a.]
PB  - Wiley-Blackwell
M1  - PreJuSER-41953
SP  - 133 - 140
PY  - 2005
N1  - Record converted from VDB: 12.11.2012
AB  - Volutin granules are intracellular storages of complexed inorganic polyphosphate (poly P). Histochemical staining procedures differentiate between pathogenic corynebacteria such as Corynebacterum diphtheriae (containing volutin) and non-pathogenic species, such as C. glutamicum. Here we report that strains ATCC13032 and MH20-22B of the non-pathogenic C. glutamicum also formed subcellular entities (18-37% of the total cell volume) that had the typical characteristics of volutin granules: (i) volutin staining, (ii) green UV fluorescence when stained with 4',6-diamidino-2-phenylindole, (iii) electron-dense and rich in phosphorus when determined with transmission electron microscopy and X-ray microanalysis, and (iv) 31P NMR poly P resonances of isolated granules dissolved in EDTA. MgCl2 addition to the growth medium stimulated granule formation but did not effect expression of genes involved in poly P metabolism. Granular volutin fractions from lysed cells contained polyphosphate glucokinase as detected by SDS-PAGE/MALDI-TOF, indicating that this poly P metabolizing enzyme is present also in intact poly P granules. The results suggest that formation of volutin is a more widespread phenomenon than generally accepted.
KW  - Bacterial Proteins: genetics
KW  - Bacterial Proteins: metabolism
KW  - Corynebacterium glutamicum: genetics
KW  - Corynebacterium glutamicum: metabolism
KW  - Corynebacterium glutamicum: ultrastructure
KW  - Cytoplasmic Granules: metabolism
KW  - Cytoplasmic Granules: ultrastructure
KW  - Electron Probe Microanalysis
KW  - Gene Expression Profiling
KW  - Gene Expression Regulation, Bacterial
KW  - Inorganic Chemicals: metabolism
KW  - Magnesium Chloride: pharmacology
KW  - Magnetic Resonance Spectroscopy
KW  - Microscopy, Electron, Transmission
KW  - Oligonucleotide Array Sequence Analysis
KW  - Phosphorus Isotopes
KW  - Phosphotransferases: metabolism
KW  - Polyphosphates: metabolism
KW  - Proteins: metabolism
KW  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: methods
KW  - Bacterial Proteins (NLM Chemicals)
KW  - Inorganic Chemicals (NLM Chemicals)
KW  - Phosphorus Isotopes (NLM Chemicals)
KW  - Polyphosphates (NLM Chemicals)
KW  - Proteins (NLM Chemicals)
KW  - Magnesium Chloride (NLM Chemicals)
KW  - Phosphotransferases (NLM Chemicals)
KW  - polyphosphate-glucose phosphotransferase (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:15668011
UR  - <Go to ISI:>//WOS:000226875000019
DO  - DOI:10.1016/j.femsle.2004.11.047
UR  - https://juser.fz-juelich.de/record/41953
ER  -