% IMPORTANT: The following is UTF-8 encoded. This means that in the presence % of non-ASCII characters, it will not work with BibTeX 0.99 or older. % Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or % “biber”. @ARTICLE{Pallerla:41953, author = {Pallerla, S. R. and Knebel, S. and Polen, T. and Klauth, P. and Hollender, J. and Wendisch, V. F. and Schoberth, S. M.}, title = {{F}ormation of {V}olutin {G}ranules in {C}orynebacterium glutamicum}, journal = {FEMS microbiology letters}, volume = {243}, issn = {0378-1097}, address = {Oxford [u.a.]}, publisher = {Wiley-Blackwell}, reportid = {PreJuSER-41953}, pages = {133 - 140}, year = {2005}, note = {Record converted from VDB: 12.11.2012}, abstract = {Volutin granules are intracellular storages of complexed inorganic polyphosphate (poly P). Histochemical staining procedures differentiate between pathogenic corynebacteria such as Corynebacterum diphtheriae (containing volutin) and non-pathogenic species, such as C. glutamicum. Here we report that strains ATCC13032 and MH20-22B of the non-pathogenic C. glutamicum also formed subcellular entities $(18-37\%$ of the total cell volume) that had the typical characteristics of volutin granules: (i) volutin staining, (ii) green UV fluorescence when stained with 4',6-diamidino-2-phenylindole, (iii) electron-dense and rich in phosphorus when determined with transmission electron microscopy and X-ray microanalysis, and (iv) 31P NMR poly P resonances of isolated granules dissolved in EDTA. MgCl2 addition to the growth medium stimulated granule formation but did not effect expression of genes involved in poly P metabolism. Granular volutin fractions from lysed cells contained polyphosphate glucokinase as detected by SDS-PAGE/MALDI-TOF, indicating that this poly P metabolizing enzyme is present also in intact poly P granules. The results suggest that formation of volutin is a more widespread phenomenon than generally accepted.}, keywords = {Bacterial Proteins: genetics / Bacterial Proteins: metabolism / Corynebacterium glutamicum: genetics / Corynebacterium glutamicum: metabolism / Corynebacterium glutamicum: ultrastructure / Cytoplasmic Granules: metabolism / Cytoplasmic Granules: ultrastructure / Electron Probe Microanalysis / Gene Expression Profiling / Gene Expression Regulation, Bacterial / Inorganic Chemicals: metabolism / Magnesium Chloride: pharmacology / Magnetic Resonance Spectroscopy / Microscopy, Electron, Transmission / Oligonucleotide Array Sequence Analysis / Phosphorus Isotopes / Phosphotransferases: metabolism / Polyphosphates: metabolism / Proteins: metabolism / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: methods / Bacterial Proteins (NLM Chemicals) / Inorganic Chemicals (NLM Chemicals) / Phosphorus Isotopes (NLM Chemicals) / Polyphosphates (NLM Chemicals) / Proteins (NLM Chemicals) / Magnesium Chloride (NLM Chemicals) / Phosphotransferases (NLM Chemicals) / polyphosphate-glucose phosphotransferase (NLM Chemicals) / J (WoSType)}, cin = {IBT-1 / ICG-IV}, ddc = {570}, cid = {I:(DE-Juel1)VDB55 / I:(DE-Juel1)VDB50}, pnm = {Biotechnologie / Chemie und Dynamik der Geo-Biosphäre}, pid = {G:(DE-Juel1)FUEK256 / G:(DE-Juel1)FUEK257}, shelfmark = {Microbiology}, typ = {PUB:(DE-HGF)16}, pubmed = {pmid:15668011}, UT = {WOS:000226875000019}, doi = {10.1016/j.femsle.2004.11.047}, url = {https://juser.fz-juelich.de/record/41953}, }