Home > Publications database > Formation of Volutin Granules in Corynebacterium glutamicum > print

001     41953
005     20190625111454.0
024 7 _ |2 pmid
|a pmid:15668011
024 7 _ |2 DOI
|a 10.1016/j.femsle.2004.11.047
024 7 _ |2 WOS
|a WOS:000226875000019
024 7 _ |a altmetric:3608115
|2 altmetric
037 _ _ |a PreJuSER-41953
041 _ _ |a eng
082 _ _ |a 570
084 _ _ |2 WoS
|a Microbiology
100 1 _ |a Pallerla, S. R.
|b 0
|u FZJ
|0 P:(DE-Juel1)VDB42664
245 _ _ |a Formation of Volutin Granules in Corynebacterium glutamicum
260 _ _ |a Oxford [u.a.]
|b Wiley-Blackwell
|c 2005
300 _ _ |a 133 - 140
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Fems Microbiology Letters
|x 0378-1097
|0 2056
|y 1
|v 243
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a Volutin granules are intracellular storages of complexed inorganic polyphosphate (poly P). Histochemical staining procedures differentiate between pathogenic corynebacteria such as Corynebacterum diphtheriae (containing volutin) and non-pathogenic species, such as C. glutamicum. Here we report that strains ATCC13032 and MH20-22B of the non-pathogenic C. glutamicum also formed subcellular entities (18-37% of the total cell volume) that had the typical characteristics of volutin granules: (i) volutin staining, (ii) green UV fluorescence when stained with 4',6-diamidino-2-phenylindole, (iii) electron-dense and rich in phosphorus when determined with transmission electron microscopy and X-ray microanalysis, and (iv) 31P NMR poly P resonances of isolated granules dissolved in EDTA. MgCl2 addition to the growth medium stimulated granule formation but did not effect expression of genes involved in poly P metabolism. Granular volutin fractions from lysed cells contained polyphosphate glucokinase as detected by SDS-PAGE/MALDI-TOF, indicating that this poly P metabolizing enzyme is present also in intact poly P granules. The results suggest that formation of volutin is a more widespread phenomenon than generally accepted.
536 _ _ |a Biotechnologie
|c L02
|2 G:(DE-HGF)
|0 G:(DE-Juel1)FUEK256
|x 0
536 _ _ |a Chemie und Dynamik der Geo-Biosphäre
|c U01
|0 G:(DE-Juel1)FUEK257
|x 1
588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Bacterial Proteins: genetics
650 _ 2 |2 MeSH
|a Bacterial Proteins: metabolism
650 _ 2 |2 MeSH
|a Corynebacterium glutamicum: genetics
650 _ 2 |2 MeSH
|a Corynebacterium glutamicum: metabolism
650 _ 2 |2 MeSH
|a Corynebacterium glutamicum: ultrastructure
650 _ 2 |2 MeSH
|a Cytoplasmic Granules: metabolism
650 _ 2 |2 MeSH
|a Cytoplasmic Granules: ultrastructure
650 _ 2 |2 MeSH
|a Electron Probe Microanalysis
650 _ 2 |2 MeSH
|a Gene Expression Profiling
650 _ 2 |2 MeSH
|a Gene Expression Regulation, Bacterial
650 _ 2 |2 MeSH
|a Inorganic Chemicals: metabolism
650 _ 2 |2 MeSH
|a Magnesium Chloride: pharmacology
650 _ 2 |2 MeSH
|a Magnetic Resonance Spectroscopy
650 _ 2 |2 MeSH
|a Microscopy, Electron, Transmission
650 _ 2 |2 MeSH
|a Oligonucleotide Array Sequence Analysis
650 _ 2 |2 MeSH
|a Phosphorus Isotopes
650 _ 2 |2 MeSH
|a Phosphotransferases: metabolism
650 _ 2 |2 MeSH
|a Polyphosphates: metabolism
650 _ 2 |2 MeSH
|a Proteins: metabolism
650 _ 2 |2 MeSH
|a Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization: methods
650 _ 7 |0 0
|2 NLM Chemicals
|a Bacterial Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a Inorganic Chemicals
650 _ 7 |0 0
|2 NLM Chemicals
|a Phosphorus Isotopes
650 _ 7 |0 0
|2 NLM Chemicals
|a Polyphosphates
650 _ 7 |0 0
|2 NLM Chemicals
|a Proteins
650 _ 7 |0 7786-30-3
|2 NLM Chemicals
|a Magnesium Chloride
650 _ 7 |0 EC 2.7.-
|2 NLM Chemicals
|a Phosphotransferases
650 _ 7 |0 EC 2.7.1.63
|2 NLM Chemicals
|a polyphosphate-glucose phosphotransferase
650 _ 7 |a J
|2 WoSType
653 2 0 |2 Author
|a Corynebacterium glutamicum
653 2 0 |2 Author
|a inorganic polyphosphate
653 2 0 |2 Author
|a volutin granules
653 2 0 |2 Author
|a electron dispersive X-ray analysis
653 2 0 |2 Author
|a DAPI fluorescence
653 2 0 |2 Author
|a P-31 NMR
653 2 0 |2 Author
|a magnesium
653 2 0 |2 Author
|a polyphosphate glucokinase
653 2 0 |2 Author
|a MALDI-TOF
653 2 0 |2 Author
|a DNA microarray
700 1 _ |a Knebel, S.
|b 1
|u FZJ
|0 P:(DE-Juel1)VDB43392
700 1 _ |a Polen, T.
|b 2
|u FZJ
|0 P:(DE-Juel1)128982
700 1 _ |a Klauth, P.
|b 3
|0 P:(DE-HGF)0
700 1 _ |a Hollender, J.
|b 4
|0 P:(DE-HGF)0
700 1 _ |a Wendisch, V. F.
|b 5
|u FZJ
|0 P:(DE-Juel1)VDB1764
700 1 _ |a Schoberth, S. M.
|b 6
|u FZJ
|0 P:(DE-Juel1)VDB10831
773 _ _ |a 10.1016/j.femsle.2004.11.047
|g Vol. 243, p. 133 - 140
|p 133 - 140
|q 243<133 - 140
|0 PERI:(DE-600)1501716-3
|t FEMS microbiology letters
|v 243
|y 2005
|x 0378-1097
856 7 _ |u http://dx.doi.org/10.1016/j.femsle.2004.11.047
909 C O |o oai:juser.fz-juelich.de:41953
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914 1 _ |y 2005
915 _ _ |0 StatID:(DE-HGF)0010
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