TY  - JOUR
AU  - Patra, D.
AU  - Gregor, I.
AU  - Enderlein, J.
TI  - Image Analysis of Defocused Single-molecule Images for Three-dimensional Molecule Orientation Studies
JO  - The journal of physical chemistry  / A
VL  - 108
SN  - 1089-5639
CY  - Washington, DC
PB  - Soc.
M1  - PreJuSER-42220
SP  - 6836 - 6841
PY  - 2004
N1  - Record converted from VDB: 12.11.2012
AB  - An efficient algorithm for pattern matching has been developed based on least-squares analysis of fitting a discrete set of master patterns against measured images. This algorithm has been applied to determine three-dimensional molecule orientations in defocused single-molecule images. The developed algorithm exploits the excellent agreement between electrodynamic calculations of single-molecule emission and experimentally measured images. The procedure is found to be reliable and simple and can be applied to any kind of pattern recognition where the patterns to be recognized are precisely known a priori. The procedure works well even for noisy and low-intensity signals as usually encountered in single-molecule experiments.
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000223289700006
DO  - DOI:10.1021/jp048188m
UR  - https://juser.fz-juelich.de/record/42220
ER  -