Image Analysis of Defocused Single-molecule Images for Three-dimensional Molecule Orientation Studies

Patra, D.; Enderlein, J.; Gregor, I.

doi:10.1021/jp048188m

Journal Article

PreJuSER-42220

Image Analysis of Defocused Single-molecule Images for Three-dimensional Molecule Orientation Studies

Patra, D.FZJ* ; Gregor, I.FZJ* ; Enderlein, J.FZJ*

2004
Soc. Washington, DC

The journal of physical chemistry <Washington, DC> / A 108, 6836 - 6841 (2004) [10.1021/jp048188m]

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Please use a persistent id in citations: http://hdl.handle.net/2128/702 doi:10.1021/jp048188m

Abstract: An efficient algorithm for pattern matching has been developed based on least-squares analysis of fitting a discrete set of master patterns against measured images. This algorithm has been applied to determine three-dimensional molecule orientations in defocused single-molecule images. The developed algorithm exploits the excellent agreement between electrodynamic calculations of single-molecule emission and experimentally measured images. The procedure is found to be reliable and simple and can be applied to any kind of pattern recognition where the patterns to be recognized are precisely known a priori. The procedure works well even for noisy and low-intensity signals as usually encountered in single-molecule experiments.

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