%0 Journal Article
%A Koenig, B. W.
%A Gawrisch, K.
%T Lipid ethanol interaction studied by NMR on bicelles
%J The journal of physical chemistry  / B
%V 109
%@ 1520-6106
%C Washington, DC
%I Soc.
%M PreJuSER-45561
%P 7540 - 7547
%D 2005
%Z Record converted from VDB: 12.11.2012
%X The interaction of ethanol with phospholipids was studied in bicelles at a physiologically relevant ethanol concentration of 20 mM and a lipid content of 14 wt % by high-resolution NMR. Transient association of ethanol with magnetically aligned bicelles imparts a small degree of anisotropy to the solute. This anisotropy allows detection of residual (1)H-(1)H and (1)H-(13)C dipolar couplings, which are superimposed on scalar couplings. Residual (2)H NMR quadrupole splittings of isotope-labeled ethanol were measured as well. The analysis of residual tensorial interactions yielded information on the orientation and motions of ethanol in the membrane-bound state. The fraction of phosphatidylcholine-bound ethanol was determined independently by gas chromatography and NMR. About 4% of ethanol is bound to phosphatidylcholine at a bicelle concentration of 14 wt % at 40 degrees C. Free and bound ethanol are in rapid exchange. The lifetime of ethanol association with phosphatidylcholine membranes is of the order of a few nanoseconds.
%K Chemistry, Physical: methods
%K Chromatography, Gas
%K Dimyristoylphosphatidylcholine: chemistry
%K Ethanol: chemistry
%K Lipid Bilayers
%K Lipids: chemistry
%K Magnetic Resonance Spectroscopy
%K Molecular Conformation
%K Phosphatidylcholines: chemistry
%K Phospholipids: chemistry
%K Time Factors
%K Lipid Bilayers (NLM Chemicals)
%K Lipids (NLM Chemicals)
%K Phosphatidylcholines (NLM Chemicals)
%K Phospholipids (NLM Chemicals)
%K Dimyristoylphosphatidylcholine (NLM Chemicals)
%K Ethanol (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:16851866
%U <Go to ISI:>//WOS:000228419100088
%R 10.1021/jp044980b
%U https://juser.fz-juelich.de/record/45561