Lipid ethanol interaction studied by NMR on bicelles

Koenig, B. W.; Gawrisch, K.

doi:10.1021/jp044980b

Journal Article

PreJuSER-45561

Lipid ethanol interaction studied by NMR on bicelles

Koenig, B. W.FZJ* ; Gawrisch, K.

2005
Soc. Washington, DC

The journal of physical chemistry <Washington, DC> / B 109, 7540 - 7547 (2005) [10.1021/jp044980b]

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Please use a persistent id in citations: http://hdl.handle.net/2128/706 doi:10.1021/jp044980b

Abstract: The interaction of ethanol with phospholipids was studied in bicelles at a physiologically relevant ethanol concentration of 20 mM and a lipid content of 14 wt % by high-resolution NMR. Transient association of ethanol with magnetically aligned bicelles imparts a small degree of anisotropy to the solute. This anisotropy allows detection of residual (1)H-(1)H and (1)H-(13)C dipolar couplings, which are superimposed on scalar couplings. Residual (2)H NMR quadrupole splittings of isotope-labeled ethanol were measured as well. The analysis of residual tensorial interactions yielded information on the orientation and motions of ethanol in the membrane-bound state. The fraction of phosphatidylcholine-bound ethanol was determined independently by gas chromatography and NMR. About 4% of ethanol is bound to phosphatidylcholine at a bicelle concentration of 14 wt % at 40 degrees C. Free and bound ethanol are in rapid exchange. The lifetime of ethanol association with phosphatidylcholine membranes is of the order of a few nanoseconds.

Keyword(s): Chemistry, Physical: methods (MeSH) ; Chromatography, Gas (MeSH) ; Dimyristoylphosphatidylcholine: chemistry (MeSH) ; Ethanol: chemistry (MeSH) ; Lipid Bilayers (MeSH) ; Lipids: chemistry (MeSH) ; Magnetic Resonance Spectroscopy (MeSH) ; Molecular Conformation (MeSH) ; Phosphatidylcholines: chemistry (MeSH) ; Phospholipids: chemistry (MeSH) ; Time Factors (MeSH) ; Lipid Bilayers ; Lipids ; Phosphatidylcholines ; Phospholipids ; Dimyristoylphosphatidylcholine ; Ethanol ; J