TY  - JOUR
AU  - Koenig, B. W.
AU  - Gawrisch, K.
TI  - Lipid ethanol interaction studied by NMR on bicelles
JO  - The journal of physical chemistry  / B
VL  - 109
SN  - 1520-6106
CY  - Washington, DC
PB  - Soc.
M1  - PreJuSER-45561
SP  - 7540 - 7547
PY  - 2005
N1  - Record converted from VDB: 12.11.2012
AB  - The interaction of ethanol with phospholipids was studied in bicelles at a physiologically relevant ethanol concentration of 20 mM and a lipid content of 14 wt % by high-resolution NMR. Transient association of ethanol with magnetically aligned bicelles imparts a small degree of anisotropy to the solute. This anisotropy allows detection of residual (1)H-(1)H and (1)H-(13)C dipolar couplings, which are superimposed on scalar couplings. Residual (2)H NMR quadrupole splittings of isotope-labeled ethanol were measured as well. The analysis of residual tensorial interactions yielded information on the orientation and motions of ethanol in the membrane-bound state. The fraction of phosphatidylcholine-bound ethanol was determined independently by gas chromatography and NMR. About 4% of ethanol is bound to phosphatidylcholine at a bicelle concentration of 14 wt % at 40 degrees C. Free and bound ethanol are in rapid exchange. The lifetime of ethanol association with phosphatidylcholine membranes is of the order of a few nanoseconds.
KW  - Chemistry, Physical: methods
KW  - Chromatography, Gas
KW  - Dimyristoylphosphatidylcholine: chemistry
KW  - Ethanol: chemistry
KW  - Lipid Bilayers
KW  - Lipids: chemistry
KW  - Magnetic Resonance Spectroscopy
KW  - Molecular Conformation
KW  - Phosphatidylcholines: chemistry
KW  - Phospholipids: chemistry
KW  - Time Factors
KW  - Lipid Bilayers (NLM Chemicals)
KW  - Lipids (NLM Chemicals)
KW  - Phosphatidylcholines (NLM Chemicals)
KW  - Phospholipids (NLM Chemicals)
KW  - Dimyristoylphosphatidylcholine (NLM Chemicals)
KW  - Ethanol (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:16851866
UR  - <Go to ISI:>//WOS:000228419100088
DO  - DOI:10.1021/jp044980b
UR  - https://juser.fz-juelich.de/record/45561
ER  -