%0 Journal Article
%A Katranidis, A.
%A Atta, D.
%A Schlesinger, R.
%A Nierhaus, K.H.
%A Choli-Papadopoulous, T.
%A Gregor, I.
%A Gerrits, M.
%A Büldt, G.
%A Fitter, J.
%T Fast biosynthesis of GFP molecules - a single molecule fluorescence study
%J Angewandte Chemie / International edition
%V 48
%@ 1521-3773
%C Weinheim
%I Wiley-VCH
%M PreJuSER-4684
%P 1758 - 1761
%D 2009
%Z Record converted from VDB: 12.11.2012
%X It's not easy being green: Real-time visualization of labeled ribosomes and de novo synthesized green fluorescent protein molecules using single-molecule-sensitive fluorescence microscopy demonstrates that the mutant GFPem is produced with a characteristic time of five minutes. Fluorescence of the fastest GFP molecules appears within one minute (see picture).
%K Fluorescence
%K Fluorescent Dyes: metabolism
%K Green Fluorescent Proteins: biosynthesis
%K Microscopy, Fluorescence
%K Protein Folding
%K Ribosomes: metabolism
%K Time Factors
%K Fluorescent Dyes (NLM Chemicals)
%K Green Fluorescent Proteins (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:19173359
%U <Go to ISI:>//WOS:000264034700008
%R 10.1002/anie.200806070
%U https://juser.fz-juelich.de/record/4684