Journal Article

PreJuSER-4684

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Fast biosynthesis of GFP molecules - a single molecule fluorescence study

Katranidis, A.FZJ* ; Atta, D.FZJ* ; Schlesinger, R.FZJ* ; Nierhaus, K. H. ; Choli-Papadopoulous, T. ; Gregor, I. ; Gerrits, M. ; Büldt, G.FZJ* ; Fitter, J.FZJ*

2009
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/anie.200806070

Abstract: It's not easy being green: Real-time visualization of labeled ribosomes and de novo synthesized green fluorescent protein molecules using single-molecule-sensitive fluorescence microscopy demonstrates that the mutant GFPem is produced with a characteristic time of five minutes. Fluorescence of the fastest GFP molecules appears within one minute (see picture).

Keyword(s): Fluorescence (MeSH) ; Fluorescent Dyes: metabolism (MeSH) ; Green Fluorescent Proteins: biosynthesis (MeSH) ; Microscopy, Fluorescence (MeSH) ; Protein Folding (MeSH) ; Ribosomes: metabolism (MeSH) ; Time Factors (MeSH) ; Fluorescent Dyes ; Green Fluorescent Proteins ; J ; fluorescence microscopy (auto) ; green fluorescent proteins (auto) ; protein folding (auto) ; ribosomes (auto) ; single-molecule studies (auto)

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Molekulare Biophysik (ISB-2)

Research Program(s):

1. Programm Biosoft (N03)

Appears in the scientific report 2009

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