TY - JOUR
AU - Katranidis, A.
AU - Atta, D.
AU - Schlesinger, R.
AU - Nierhaus, K.H.
AU - Choli-Papadopoulous, T.
AU - Gregor, I.
AU - Gerrits, M.
AU - Büldt, G.
AU - Fitter, J.
TI - Fast biosynthesis of GFP molecules - a single molecule fluorescence study
JO - Angewandte Chemie / International edition
VL - 48
SN - 1521-3773
CY - Weinheim
PB - Wiley-VCH
M1 - PreJuSER-4684
SP - 1758 - 1761
PY - 2009
N1 - Record converted from VDB: 12.11.2012
AB - It's not easy being green: Real-time visualization of labeled ribosomes and de novo synthesized green fluorescent protein molecules using single-molecule-sensitive fluorescence microscopy demonstrates that the mutant GFPem is produced with a characteristic time of five minutes. Fluorescence of the fastest GFP molecules appears within one minute (see picture).
KW - Fluorescence
KW - Fluorescent Dyes: metabolism
KW - Green Fluorescent Proteins: biosynthesis
KW - Microscopy, Fluorescence
KW - Protein Folding
KW - Ribosomes: metabolism
KW - Time Factors
KW - Fluorescent Dyes (NLM Chemicals)
KW - Green Fluorescent Proteins (NLM Chemicals)
KW - J (WoSType)
LB - PUB:(DE-HGF)16
C6 - pmid:19173359
UR - <Go to ISI:>//WOS:000264034700008
DO - DOI:10.1002/anie.200806070
UR - https://juser.fz-juelich.de/record/4684
ER -