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000047386 041__ $$aeng
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000047386 084__ $$2WoS$$aChemistry, Multidisciplinary
000047386 084__ $$2WoS$$aChemistry, Physical
000047386 084__ $$2WoS$$aMaterials Science, Multidisciplinary
000047386 1001_ $$0P:(DE-Juel1)VDB7601$$aRatanabanangkoon, P.$$b0$$uFZJ
000047386 245__ $$aTwo-dimensional streptavidin crystals on giant lipid bilayer vesicles
000047386 260__ $$aWashington, DC$$bACS Publ.$$c2002
000047386 300__ $$a4270 - 4276
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000047386 520__ $$aStreptavidin was crystallized on giant bilayer vesicles (20-60 mum) in sucrose solution at various pH values. The streptavidin-coated vesicles exhibited unique roughened spherical and prolate ellipsoidal shapes, illustrating resistance to curvature of the two-dimensional crystals. Studies indicated that the spheroids and prolate ellipsoids correspond to different crystal morphologies. Through confocal microscopy, the various crystal morphologies on vesicle surfaces were observed under different solution conditions. Unlike two-dimensional (2D) streptavidin crystals grown in ionic buffer that assume the P1, P2, and C222 lattices at pH 4, 5.5, and 7, respectively (Wang et al. Langmuir 1999, 15, 154 1), crystals grown in sucrose with no added salt show only the lowest density C222 lattice due to strong electrostatic interactions.
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000047386 7001_ $$0P:(DE-Juel1)VDB7602$$aGropper, M.$$b1$$uFZJ
000047386 7001_ $$0P:(DE-Juel1)128833$$aMerkel, R.$$b2$$uFZJ
000047386 7001_ $$0P:(DE-Juel1)VDB7604$$aSackmann, M. J.$$b3$$uFZJ
000047386 7001_ $$0P:(DE-Juel1)VDB7605$$aGast, A. P.$$b4$$uFZJ
000047386 773__ $$0PERI:(DE-600)2005937-1$$a10.1021/la025568v$$gVol. 18, p. 4270 - 4276$$p4270 - 4276$$q18<4270 - 4276$$tLangmuir$$v18$$x0743-7463$$y2002
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