Journal Article

PreJuSER-47386

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Two-dimensional streptavidin crystals on giant lipid bilayer vesicles

Ratanabanangkoon, P.FZJ* ; Gropper, M.FZJ* ; Merkel, R.FZJ* ; Sackmann, M. J.FZJ* ; Gast, A. P.FZJ*

2002
ACS Publ. Washington, DC

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Please use a persistent id in citations: http://hdl.handle.net/2128/2179  doi:10.1021/la025568v

Abstract: Streptavidin was crystallized on giant bilayer vesicles (20-60 mum) in sucrose solution at various pH values. The streptavidin-coated vesicles exhibited unique roughened spherical and prolate ellipsoidal shapes, illustrating resistance to curvature of the two-dimensional crystals. Studies indicated that the spheroids and prolate ellipsoids correspond to different crystal morphologies. Through confocal microscopy, the various crystal morphologies on vesicle surfaces were observed under different solution conditions. Unlike two-dimensional (2D) streptavidin crystals grown in ionic buffer that assume the P1, P2, and C222 lattices at pH 4, 5.5, and 7, respectively (Wang et al. Langmuir 1999, 15, 154 1), crystals grown in sucrose with no added salt show only the lowest density C222 lattice due to strong electrostatic interactions.

Keyword(s): J

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Institut für biologisch-anorganische Grenzflächen (ISG-4)

Research Program(s):

1. Kondensierte Materie (M02)

Appears in the scientific report 2002

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Database coverage:

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