TY  - JOUR
AU  - Ratanabanangkoon, P.
AU  - Gropper, M.
AU  - Merkel, R.
AU  - Sackmann, M. J.
AU  - Gast, A. P.
TI  - Two-dimensional streptavidin crystals on giant lipid bilayer vesicles
JO  - Langmuir
VL  - 18
SN  - 0743-7463
CY  - Washington, DC
PB  - ACS Publ.
M1  - PreJuSER-47386
SP  - 4270 - 4276
PY  - 2002
N1  - Record converted from VDB: 12.11.2012
AB  - Streptavidin was crystallized on giant bilayer vesicles (20-60 mum) in sucrose solution at various pH values. The streptavidin-coated vesicles exhibited unique roughened spherical and prolate ellipsoidal shapes, illustrating resistance to curvature of the two-dimensional crystals. Studies indicated that the spheroids and prolate ellipsoids correspond to different crystal morphologies. Through confocal microscopy, the various crystal morphologies on vesicle surfaces were observed under different solution conditions. Unlike two-dimensional (2D) streptavidin crystals grown in ionic buffer that assume the P1, P2, and C222 lattices at pH 4, 5.5, and 7, respectively (Wang et al. Langmuir 1999, 15, 154 1), crystals grown in sucrose with no added salt show only the lowest density C222 lattice due to strong electrostatic interactions.
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000175801600016
DO  - DOI:10.1021/la025568v
UR  - https://juser.fz-juelich.de/record/47386
ER  -