Journal Article

PreJuSER-4813

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png ITS2 sequence-structure phylogeny in the Scenedesmaceae with special reference to Coelastrum (Chlorophyta, Chlorophyceae), including the new genera Comasiella and Pectinodesmus

Hegewald, E.FZJ* ; Wolf, M. ; Keller, A. ; Friedl, T. ; Krienitz, L.

2010
Soc. Lawrence, KS

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Please use a persistent id in citations: doi:10.2216/09-61.1

Abstract: Sequences and secondary structures of the nuclear-encoded internal transcribed spacer 2 (ITS2) ribosomal RNA of nine Coelastrum taxa, Asterarcys quadricellulare (Coelastraceae). Westella botryoides (hitherto Oocystaceae) and Dimorphococcus lunatus (Scenedesmaceae) were determined and compared with existing GenBank entries of scenedesmacean taxa (Desmodesmus, Enallax, Neodesmus Scenedesmus). Phylogenetic analyses showed that the studied Coelastrum taxa belong to several different lineages within the Scenedesmaceae: five Coelastrum taxa (Coelastrum microporum, Coelastrum astroideum, C. astroideum var. rugosum = Coelastrum rugosum, Coelastrum pseudomicroporum and Coelastrum sphaericum incl. Coelastrum proboscideum) form monophyletic clades, whereas two strains labeled Coelastrum morum belong to different genera. The African strain of C. martini clusters with Coelastrum cambricum. The Finnish strain labeled C. morum clusters with Asterarcys, Dimorphococcus and Hariotina. According to its morphology this strain belongs to Coelastrella, related to Coelastrella saiponensis. Westella botryoides belongs to a separate elude within the Scenedesmaceae. Coelastrum reticulatum is positioned in the chide with Asterarcys, Dimorphococcus and Coelastrella; hence its separation in a separate genus, as originally described (Hariotina), is justified. In general, the phylogenetic analysis of ITS2 data shows that the Coelastraceae are included in the monophyletic Scenedesmaceae, and thus the splitting into two families is not justified, but they belong to the monophyletic subfamily Coelastroidea. The genera Comasiella and Pectinodesmus are newly erected, and several new combinations are proposed.

Keyword(s): J ; ITS2 (auto) ; new combinations (auto) ; phylogeny (auto) ; secondary structure (auto) ; Acutodesmus (auto) ; Coelastraceae (auto) ; Coelastrella (auto) ; Coelastrum (auto) ; Comasiella gen. nov. (auto) ; Hariotina (auto) ; Pectinodesmus gen. nov. (auto) ; Scenedesmaceae (auto) ; Scenedesmus (auto) ; Westella (auto)

Note: We thank L. Wei for the alignment of strain Krienitz 2005-45, D. Hepperle for the alignments of six strains and T. Holtmann for translating the diagnosis into Latin language. We thank W. Schroeder (Figs 2-7, 11-13) and H.-P. Bochem for SEM photos (Figs 15-17) and the reviewers and the associate editor for valuable comments and language corrections. L. Krienitz and M. Wolf were supported by grants of the Deutsche Forschungsgemeinschaft (DFG). A. Keller acknowledges the support of the BIGSS graduate school. This work was supported by a grant extended by the German Ministry of Education and Research (BMBF) to TF, contract 01LC0026, project BIOLOG 'AlgaTerra AT4'.

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Research Program(s):

1. Terrestrische Umwelt (P24)

Appears in the scientific report 2010

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