Journal Article

PreJuSER-48349

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Statistical analysis of diffusion coefficient determination by fluorescence correlation spectroscopy

Enderlein, J.FZJ* ; Gregor, I.FZJ* ; Patra, D. ; Fitter, J.FZJ*

2005
Springer Science + Business Media B.V. New York, NY

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Please use a persistent id in citations: doi:10.1007/s10895-005-2633-0

Abstract: Fluorescence correlation spectroscopy (FCS) has become an important and widely used technique for many applications in physics, chemistry, and biology. The parameter most frequently addressed by FCS is the diffusion of molecules in solution. Due to the highly non-linear connection between the diffusion coefficient and a measured autocorrelation function, it is extremely difficult to analyse the accuracy of the diffusion-coefficient determination in a FCS experiment. Here, we present a simplified analysis based on some general maximum-likelihood considerations, and numerical result are given for the dependence of the accuracy of the diffusion-coefficient determination on sample concentration, brightness, and measurement time. Optimal concentration values for performing FCS are found.

Keyword(s): J ; fluorescence correlation spectroscopy (auto) ; diffusion coefficient (auto) ; statistical accuracy (auto)

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Zelluläre Signalverarbeitung (IBI-1)
2. Biologische Strukturforschung (IBI-2)

Research Program(s):

1. Neurowissenschaften (L01)

Appears in the scientific report 2005

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