%0 Journal Article
%A Atanasov, V.
%A Knorr, N.
%A Duran, R. S.
%A Ingebrandt, S.
%A Offenhäusser, A.
%A Knoll, W.
%A Köper, I.
%T Membrane on a Chip. A functional tethered lipid bilayer membrane on silicon oxide surface
%J Biophysical journal
%V 89
%@ 0006-3495
%C New York, NY
%I Rockefeller Univ. Press
%M PreJuSER-48738
%P 1780 - 1788
%D 2005
%Z Record converted from VDB: 12.11.2012
%X Tethered membranes have been proven during recent years to be a powerful and flexible biomimetic platform. We reported in a previous article on the design of a new architecture based on the self-assembly of a thiolipid on ultrasmooth gold substrates, which shows extremely good electrical sealing properties as well as functionality of a bilayer membrane. Here, we describe the synthesis of lipids for a more modular design and the adaptation of the linker part to silane chemistry. We were able to form a functional tethered bilayer lipid membrane with good electrical sealing properties covering a silicon oxide surface. We demonstrate the functional incorporation of the ion carrier valinomycin and of the ion channel gramicidin.
%K Biophysics: methods
%K Electric Impedance
%K Electrochemistry
%K Gold: chemistry
%K Gramicidin: pharmacology
%K Ion Channels: chemistry
%K Ionophores: pharmacology
%K Ions
%K Lipid Bilayers: chemistry
%K Lipid Bilayers: metabolism
%K Lipids: chemistry
%K Microscopy, Atomic Force
%K Models, Chemical
%K Phosphatidylcholines: chemistry
%K Phytol: chemistry
%K Protein Array Analysis: methods
%K Silanes: chemistry
%K Silanes: metabolism
%K Silicon: chemistry
%K Silicon Dioxide: chemistry
%K Spectrophotometry
%K Substrate Specificity
%K Temperature
%K Time Factors
%K Valinomycin: chemistry
%K Valinomycin: pharmacology
%K 2,3-di-O-phytanyl-sn-glycerol-1-tetraethylene glycol-(3-chloro-dimethylpropyl-silane) ether lipid (NLM Chemicals)
%K 2,3-di-O-phytanyl-sn-glycerol-1-tetraethylene glycol-(3-trichloropropyl-silane) ether lipid (NLM Chemicals)
%K Ion Channels (NLM Chemicals)
%K Ionophores (NLM Chemicals)
%K Ions (NLM Chemicals)
%K Lipid Bilayers (NLM Chemicals)
%K Lipids (NLM Chemicals)
%K Phosphatidylcholines (NLM Chemicals)
%K Silanes (NLM Chemicals)
%K Gramicidin (NLM Chemicals)
%K Phytol (NLM Chemicals)
%K Valinomycin (NLM Chemicals)
%K 1,2-diphytanoylphosphatidylcholine (NLM Chemicals)
%K Silicon (NLM Chemicals)
%K Gold (NLM Chemicals)
%K Silicon Dioxide (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:16127170
%2 pmc:PMC1366681
%U <Go to ISI:>//WOS:000231502800034
%R 10.1529/biophysj.105.061374
%U https://juser.fz-juelich.de/record/48738