TY  - JOUR
AU  - Atanasov, V.
AU  - Knorr, N.
AU  - Duran, R. S.
AU  - Ingebrandt, S.
AU  - Offenhäusser, A.
AU  - Knoll, W.
AU  - Köper, I.
TI  - Membrane on a Chip. A functional tethered lipid bilayer membrane on silicon oxide surface
JO  - Biophysical journal
VL  - 89
SN  - 0006-3495
CY  - New York, NY
PB  - Rockefeller Univ. Press
M1  - PreJuSER-48738
SP  - 1780 - 1788
PY  - 2005
N1  - Record converted from VDB: 12.11.2012
AB  - Tethered membranes have been proven during recent years to be a powerful and flexible biomimetic platform. We reported in a previous article on the design of a new architecture based on the self-assembly of a thiolipid on ultrasmooth gold substrates, which shows extremely good electrical sealing properties as well as functionality of a bilayer membrane. Here, we describe the synthesis of lipids for a more modular design and the adaptation of the linker part to silane chemistry. We were able to form a functional tethered bilayer lipid membrane with good electrical sealing properties covering a silicon oxide surface. We demonstrate the functional incorporation of the ion carrier valinomycin and of the ion channel gramicidin.
KW  - Biophysics: methods
KW  - Electric Impedance
KW  - Electrochemistry
KW  - Gold: chemistry
KW  - Gramicidin: pharmacology
KW  - Ion Channels: chemistry
KW  - Ionophores: pharmacology
KW  - Ions
KW  - Lipid Bilayers: chemistry
KW  - Lipid Bilayers: metabolism
KW  - Lipids: chemistry
KW  - Microscopy, Atomic Force
KW  - Models, Chemical
KW  - Phosphatidylcholines: chemistry
KW  - Phytol: chemistry
KW  - Protein Array Analysis: methods
KW  - Silanes: chemistry
KW  - Silanes: metabolism
KW  - Silicon: chemistry
KW  - Silicon Dioxide: chemistry
KW  - Spectrophotometry
KW  - Substrate Specificity
KW  - Temperature
KW  - Time Factors
KW  - Valinomycin: chemistry
KW  - Valinomycin: pharmacology
KW  - 2,3-di-O-phytanyl-sn-glycerol-1-tetraethylene glycol-(3-chloro-dimethylpropyl-silane) ether lipid (NLM Chemicals)
KW  - 2,3-di-O-phytanyl-sn-glycerol-1-tetraethylene glycol-(3-trichloropropyl-silane) ether lipid (NLM Chemicals)
KW  - Ion Channels (NLM Chemicals)
KW  - Ionophores (NLM Chemicals)
KW  - Ions (NLM Chemicals)
KW  - Lipid Bilayers (NLM Chemicals)
KW  - Lipids (NLM Chemicals)
KW  - Phosphatidylcholines (NLM Chemicals)
KW  - Silanes (NLM Chemicals)
KW  - Gramicidin (NLM Chemicals)
KW  - Phytol (NLM Chemicals)
KW  - Valinomycin (NLM Chemicals)
KW  - 1,2-diphytanoylphosphatidylcholine (NLM Chemicals)
KW  - Silicon (NLM Chemicals)
KW  - Gold (NLM Chemicals)
KW  - Silicon Dioxide (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:16127170
C2  - pmc:PMC1366681
UR  - <Go to ISI:>//WOS:000231502800034
DO  - DOI:10.1529/biophysj.105.061374
UR  - https://juser.fz-juelich.de/record/48738
ER  -