TY  - JOUR
AU  - Guo, H.
AU  - Kottke, T.
AU  - Hegemann, P.
AU  - Dick, B.
TI  - The Phot LOLV2 Domain and its Interaction with LOV1
JO  - Biophysical journal
VL  - 89
SN  - 0006-3495
CY  - New York, NY
PB  - Rockefeller Univ. Press
M1  - PreJuSER-49345
SP  - 402 - 412
PY  - 2005
N1  - Record converted from VDB: 12.11.2012
AB  - Phot proteins are homologs of the blue-light receptor phototropin. We report a comparative study of the photocycles of the isolated, light-sensitive domains LOV1 and LOV2 from Chlamydomonas reinhardtii phot protein, as well as the construct LOV1/2 containing both domains. Transient absorption measurements revealed a short lifetime of the LOV2-wt triplet state (500 ns), but a long lifetime (287 micros) of the triplet in the mutant LOV2-C250S, in which the reactive cysteine is replaced by serine. For LOV1, in comparison, corresponding numbers of 800 ns and 4 micros for the two conformers in LOV1-wt, and 27 micros for LOV1-C57S have been reported. The triplet decay kinetics in the mixed domains LOV1/2-wt, LOV1/2-C57S, and LOV1/2-C250S can be analyzed as the superposition of the behavior of the corresponding single domains. The situation is different for the slow, thermal reaction of the photoadduct back to the dark form. Whereas the individual domains LOV1 and LOV2 show two decay components, the double domains LOV1/2-C57S and LOV1/2-C250S both show only a single component. The interaction of the two domains does therefore not manifest itself during the lifetime of the triplet states, but changes the decay behavior of the adduct states.
KW  - Animals
KW  - Anions
KW  - Cations
KW  - Chlamydomonas reinhardtii: metabolism
KW  - Cryptochromes
KW  - Cysteine: chemistry
KW  - DNA Adducts
KW  - Flavoproteins: chemistry
KW  - Hot Temperature
KW  - Hydrogen-Ion Concentration
KW  - Ions
KW  - Kinetics
KW  - Light
KW  - Models, Chemical
KW  - Mutation
KW  - Photons
KW  - Photoreceptor Cells, Invertebrate: chemistry
KW  - Protein Binding
KW  - Protein Structure, Tertiary
KW  - Serine: chemistry
KW  - Sodium Chloride: pharmacology
KW  - Spectrophotometry
KW  - Temperature
KW  - Time Factors
KW  - Anions (NLM Chemicals)
KW  - Cations (NLM Chemicals)
KW  - Cryptochromes (NLM Chemicals)
KW  - DNA Adducts (NLM Chemicals)
KW  - Flavoproteins (NLM Chemicals)
KW  - Ions (NLM Chemicals)
KW  - Cysteine (NLM Chemicals)
KW  - Serine (NLM Chemicals)
KW  - Sodium Chloride (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:15879473
C2  - pmc:PMC1366540
UR  - <Go to ISI:>//WOS:000230114500043
DO  - DOI:10.1529/biophysj.104.058230
UR  - https://juser.fz-juelich.de/record/49345
ER  -