Journal Article

PreJuSER-49345

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png The Phot LOLV2 Domain and its Interaction with LOV1

Guo, H. ; Kottke, T.FZJ* ; Hegemann, P. ; Dick, B.

2005
Rockefeller Univ. Press New York, NY

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Please use a persistent id in citations: http://hdl.handle.net/2128/708  doi:10.1529/biophysj.104.058230

Abstract: Phot proteins are homologs of the blue-light receptor phototropin. We report a comparative study of the photocycles of the isolated, light-sensitive domains LOV1 and LOV2 from Chlamydomonas reinhardtii phot protein, as well as the construct LOV1/2 containing both domains. Transient absorption measurements revealed a short lifetime of the LOV2-wt triplet state (500 ns), but a long lifetime (287 micros) of the triplet in the mutant LOV2-C250S, in which the reactive cysteine is replaced by serine. For LOV1, in comparison, corresponding numbers of 800 ns and 4 micros for the two conformers in LOV1-wt, and 27 micros for LOV1-C57S have been reported. The triplet decay kinetics in the mixed domains LOV1/2-wt, LOV1/2-C57S, and LOV1/2-C250S can be analyzed as the superposition of the behavior of the corresponding single domains. The situation is different for the slow, thermal reaction of the photoadduct back to the dark form. Whereas the individual domains LOV1 and LOV2 show two decay components, the double domains LOV1/2-C57S and LOV1/2-C250S both show only a single component. The interaction of the two domains does therefore not manifest itself during the lifetime of the triplet states, but changes the decay behavior of the adduct states.

Keyword(s): Animals (MeSH) ; Anions (MeSH) ; Cations (MeSH) ; Chlamydomonas reinhardtii: metabolism (MeSH) ; Cryptochromes (MeSH) ; Cysteine: chemistry (MeSH) ; DNA Adducts (MeSH) ; Flavoproteins: chemistry (MeSH) ; Hot Temperature (MeSH) ; Hydrogen-Ion Concentration (MeSH) ; Ions (MeSH) ; Kinetics (MeSH) ; Light (MeSH) ; Models, Chemical (MeSH) ; Mutation (MeSH) ; Photons (MeSH) ; Photoreceptor Cells, Invertebrate: chemistry (MeSH) ; Protein Binding (MeSH) ; Protein Structure, Tertiary (MeSH) ; Serine: chemistry (MeSH) ; Sodium Chloride: pharmacology (MeSH) ; Spectrophotometry (MeSH) ; Temperature (MeSH) ; Time Factors (MeSH) ; Anions ; Cations ; Cryptochromes ; DNA Adducts ; Flavoproteins ; Ions ; Cysteine ; Serine ; Sodium Chloride ; J

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Biologische Strukturforschung (IBI-2)

Research Program(s):

1. Neurowissenschaften (L01)

Appears in the scientific report 2005
Notes: This version is available at the following Publisher URL: http://www.biophysj.org/

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