Home > Publications database > The Phot LOLV2 Domain and its Interaction with LOV1 > print

001     49345
005     20200423204257.0
017 _ _ |a This version is available at the following Publisher URL: http://www.biophysj.org/
024 7 _ |a pmid:15879473
|2 pmid
024 7 _ |a pmc:PMC1366540
|2 pmc
024 7 _ |a 10.1529/biophysj.104.058230
|2 DOI
024 7 _ |a WOS:000230114500043
|2 WOS
024 7 _ |a 2128/708
|2 Handle
037 _ _ |a PreJuSER-49345
041 _ _ |a eng
082 _ _ |a 570
084 _ _ |2 WoS
|a Biophysics
100 1 _ |a Guo, H.
|b 0
|0 P:(DE-HGF)0
245 _ _ |a The Phot LOLV2 Domain and its Interaction with LOV1
260 _ _ |a New York, NY
|b Rockefeller Univ. Press
|c 2005
300 _ _ |a 402 - 412
336 7 _ |a Journal Article
|0 PUB:(DE-HGF)16
|2 PUB:(DE-HGF)
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|0 0
|2 EndNote
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a article
|2 DRIVER
440 _ 0 |a Biophysical Journal
|x 0006-3495
|0 882
|v 89
500 _ _ |a Record converted from VDB: 12.11.2012
520 _ _ |a Phot proteins are homologs of the blue-light receptor phototropin. We report a comparative study of the photocycles of the isolated, light-sensitive domains LOV1 and LOV2 from Chlamydomonas reinhardtii phot protein, as well as the construct LOV1/2 containing both domains. Transient absorption measurements revealed a short lifetime of the LOV2-wt triplet state (500 ns), but a long lifetime (287 micros) of the triplet in the mutant LOV2-C250S, in which the reactive cysteine is replaced by serine. For LOV1, in comparison, corresponding numbers of 800 ns and 4 micros for the two conformers in LOV1-wt, and 27 micros for LOV1-C57S have been reported. The triplet decay kinetics in the mixed domains LOV1/2-wt, LOV1/2-C57S, and LOV1/2-C250S can be analyzed as the superposition of the behavior of the corresponding single domains. The situation is different for the slow, thermal reaction of the photoadduct back to the dark form. Whereas the individual domains LOV1 and LOV2 show two decay components, the double domains LOV1/2-C57S and LOV1/2-C250S both show only a single component. The interaction of the two domains does therefore not manifest itself during the lifetime of the triplet states, but changes the decay behavior of the adduct states.
536 _ _ |a Neurowissenschaften
|c L01
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588 _ _ |a Dataset connected to Web of Science, Pubmed
650 _ 2 |2 MeSH
|a Animals
650 _ 2 |2 MeSH
|a Anions
650 _ 2 |2 MeSH
|a Cations
650 _ 2 |2 MeSH
|a Chlamydomonas reinhardtii: metabolism
650 _ 2 |2 MeSH
|a Cryptochromes
650 _ 2 |2 MeSH
|a Cysteine: chemistry
650 _ 2 |2 MeSH
|a DNA Adducts
650 _ 2 |2 MeSH
|a Flavoproteins: chemistry
650 _ 2 |2 MeSH
|a Hot Temperature
650 _ 2 |2 MeSH
|a Hydrogen-Ion Concentration
650 _ 2 |2 MeSH
|a Ions
650 _ 2 |2 MeSH
|a Kinetics
650 _ 2 |2 MeSH
|a Light
650 _ 2 |2 MeSH
|a Models, Chemical
650 _ 2 |2 MeSH
|a Mutation
650 _ 2 |2 MeSH
|a Photons
650 _ 2 |2 MeSH
|a Photoreceptor Cells, Invertebrate: chemistry
650 _ 2 |2 MeSH
|a Protein Binding
650 _ 2 |2 MeSH
|a Protein Structure, Tertiary
650 _ 2 |2 MeSH
|a Serine: chemistry
650 _ 2 |2 MeSH
|a Sodium Chloride: pharmacology
650 _ 2 |2 MeSH
|a Spectrophotometry
650 _ 2 |2 MeSH
|a Temperature
650 _ 2 |2 MeSH
|a Time Factors
650 _ 7 |0 0
|2 NLM Chemicals
|a Anions
650 _ 7 |0 0
|2 NLM Chemicals
|a Cations
650 _ 7 |0 0
|2 NLM Chemicals
|a Cryptochromes
650 _ 7 |0 0
|2 NLM Chemicals
|a DNA Adducts
650 _ 7 |0 0
|2 NLM Chemicals
|a Flavoproteins
650 _ 7 |0 0
|2 NLM Chemicals
|a Ions
650 _ 7 |0 52-90-4
|2 NLM Chemicals
|a Cysteine
650 _ 7 |0 56-45-1
|2 NLM Chemicals
|a Serine
650 _ 7 |0 7647-14-5
|2 NLM Chemicals
|a Sodium Chloride
650 _ 7 |a J
|2 WoSType
700 1 _ |a Kottke, T.
|b 1
|u FZJ
|0 P:(DE-Juel1)VDB16784
700 1 _ |a Hegemann, P.
|b 2
|0 P:(DE-HGF)0
700 1 _ |a Dick, B.
|b 3
|0 P:(DE-HGF)0
773 _ _ |a 10.1529/biophysj.104.058230
|g Vol. 89, p. 402 - 412
|p 402 - 412
|q 89<402 - 412
|0 PERI:(DE-600)1477214-0
|t Biophysical journal
|v 89
|y 2005
|x 0006-3495
856 7 _ |2 Pubmed Central
|u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1366540
856 4 _ |u https://juser.fz-juelich.de/record/49345/files/77245.pdf
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913 1 _ |k L01
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|l Funktion und Dysfunktion des Nervensystems
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914 1 _ |y 2005
915 _ _ |0 StatID:(DE-HGF)0010
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|d 31.12.2006
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