Polymerunterstützte Synthese von Oligosacchariden in homogener flüssiger Phase

Brinkmann, Nils

Dissertation / PhD Thesis/Book

Polymerunterstützte Synthese von Oligosacchariden in homogener flüssiger Phase

Brinkmann, N. (Corresponding author)FZJ*

2002
Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag Jülich

Jülich : Forschungszentrum Jülich GmbH Zentralbibliothek, Verlag, Berichte des Forschungszentrums Jülich 3990, XIII, 178 p. (2002) = Bonn, Univ., Diss., 2002

Please use a persistent id in citations: http://hdl.handle.net/2128/112

Report No.: Juel-3990

Abstract: Oligosaccharides fulfil important purposes in biological processes, which exceeds by far the role of giving stability, storing energy and so on. The transfer of information by oligosaccharides plays e. g. in infections, fertilization, inflammations, folding of proteins or cell metastasis a crucial role. It is obvious that those structures can be used as drugs. Today, in most cases the cheap provision with sufficient amounts of defined oligosaccharides is still a problem. Besides the challenge of the synthesis itself, there is often the handicap of laborious and expensive column chromatography. In this thesis the approach of polyethylene glycol supported liquid phase synthesis of oligosaccharides was pursued. For purification $\textit{state of the art}$ ultrafiltration by ceramic membranes was used, allowing to work as well in organic as in aqueous media. One model [Abb. Polymer bound Galili trisaccaride as model system] system was the synthesis of $\omega$-methoxy polyethylene glycol bound so called Galili trisaccharide Gal$\alpha$-1,3-Gal$\beta$-1,4-GlcNAc. For providing the expensive activated sugar UDP-Galactose a regeneration system was used, meaning that the galactose unit of the target was finally supported by cheap sucrose. The present work comprises among others $\bullet$ membrane techniques for the retention of polymer supported oligosaccharides $\bullet$ the use of linkers for the reversible polymer linkage $\bullet$ the provision of $\beta$-1,4-galactosyltransferase $\bullet$ a chemical and an enzymatic polymer supported oligosaccharide synthesis $\bullet$ the establishment of a technique for the $\textit{direct}$ analysis of polymer supported molecules.

Note: Record converted from VDB: 12.11.2012
Note: Bonn, Univ., Diss., 2002

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