TY  - JOUR
AU  - Stadler, A.M.
AU  - Embs, J.P.
AU  - Digel, I.
AU  - Artmann, G.M.
AU  - Unruh, T.
AU  - Büldt, G.
AU  - Zaccai, G.
TI  - Cytoplasmic Water and Hydration Layer Dynamics in Human Red Blood Cells
JO  - Journal of the American Chemical Society
VL  - 130
IS  - 50
SN  - 0002-7863
CY  - Washington, DC
PB  - American Chemical Society
M1  - PreJuSER-5171
SP  - 16852 - 16853
PY  - 2008
N1  - We thank Franz Dernmel for help with the experiment on IRIS, Aysegul Temiz Artmann and Dariusz Porst for help during sample preparation, and Moeava Tehei and Marion Jasnin for fruitful discussions. This work is based on experiments performed at the neutron sources SINQ FRM II and ISIS. This research project has been supported by the European Commission under the sixth Framework Programme through the Key Action: Strengthening the European Research Area, Research Infrastructures. Contract No: RII3-CT-2003-505925.
AB  - The dynamics of water in human red blood cells was measured with quasielastic incoherent neutron scattering in the temperature range between 290 and 320 K. Neutron spectrometers with time resolutions of 40, 13, and 7 ps were combined to cover time scales of bulk water dynamics to reduced mobility interfacial water motions. A major fraction of approximately 90% of cell water is characterized by a translational diffusion coefficient similar to bulk water. A minor fraction of approximately 10% of cellular water exhibits reduced dynamics. This slow water fraction was attributed to dynamically bound water on the surface of hemoglobin which accounts for approximately half of the hydration layer.
KW  - Cytoplasm: chemistry
KW  - Erythrocytes: chemistry
KW  - Humans
KW  - Water: chemistry
KW  - Water (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19053467
UR  - <Go to ISI:>//WOS:000263320400014
DO  - DOI:10.1021/ja807691j
UR  - https://juser.fz-juelich.de/record/5171
ER  -