Journal Article

PreJuSER-5171

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Cytoplasmic Water and Hydration Layer Dynamics in Human Red Blood Cells

Stadler, A. M.FZJ* ; Embs, J. P. ; Digel, I. ; Artmann, G. M. ; Unruh, T. ; Büldt, G.FZJ* ; Zaccai, G.

2008
American Chemical Society Washington, DC

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Please use a persistent id in citations: doi:10.1021/ja807691j

Abstract: The dynamics of water in human red blood cells was measured with quasielastic incoherent neutron scattering in the temperature range between 290 and 320 K. Neutron spectrometers with time resolutions of 40, 13, and 7 ps were combined to cover time scales of bulk water dynamics to reduced mobility interfacial water motions. A major fraction of approximately 90% of cell water is characterized by a translational diffusion coefficient similar to bulk water. A minor fraction of approximately 10% of cellular water exhibits reduced dynamics. This slow water fraction was attributed to dynamically bound water on the surface of hemoglobin which accounts for approximately half of the hydration layer.

Keyword(s): Cytoplasm: chemistry (MeSH) ; Erythrocytes: chemistry (MeSH) ; Humans (MeSH) ; Water: chemistry (MeSH) ; Water ; J

Note: We thank Franz Dernmel for help with the experiment on IRIS, Aysegul Temiz Artmann and Dariusz Porst for help during sample preparation, and Moeava Tehei and Marion Jasnin for fruitful discussions. This work is based on experiments performed at the neutron sources SINQ FRM II and ISIS. This research project has been supported by the European Commission under the sixth Framework Programme through the Key Action: Strengthening the European Research Area, Research Infrastructures. Contract No: RII3-CT-2003-505925.

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Contributing Institute(s):

1. Molekulare Biophysik (ISB-2)

Research Program(s):

1. Programm Biosoft (N03)

Experiment(s):

1. TOFTOF: Cold neutron time-of-flight spectrometer (NL2au)

Appears in the scientific report 2008
Notes: Nachtrag

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