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000052955 084__ $$2WoS$$aBiotechnology & Applied Microbiology
000052955 1001_ $$0P:(DE-Juel1)VDB764$$aKlauth, P.$$b0$$uFZJ
000052955 245__ $$aDetermination of soluble and granular inorganic  polyphosphate in Corynebacterium glutamicum
000052955 260__ $$aBerlin$$bSpringer$$c2006
000052955 300__ $$a1099 - 1106
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000052955 440_0 $$0555$$aApplied Microbiology and Biotechnology$$v72$$x0175-7598$$y5
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000052955 520__ $$aCorynebacterium glutamicum forms inorganic polyphosphate (poly P) that may occur as soluble (cytosolic) poly P and/or as volutin granules. A suitable method for monitoring soluble and granular poly P in C. glutamicum was developed and applied to C. glutamicum cells cultivated under different growth conditions. Under phosphate-limiting conditions, C. glutamicum did not accumulate poly P, but it rebuilt its poly P storages when phosphate became available. The poly P content of C. glutamicum growing on glucose minimal medium with sufficient phosphate varied considerably during growth. While the poly P content was minimal in the midexponential growth phase, two maxima were observed in the early exponential growth phase and at entry into the stationary growth phase. Cells in the early exponential growth phase primarily contained granular poly P, while cells entering the stationary growth phase contained soluble, cytosolic poly P. These results and those obtained for C. glutamicum cells cultivated under hypo- or hyperosmotic conditions or during glutamate production revealed that the poly P content of C. glutamicum and the partitioning between cytosolic and granular forms of poly P are dynamics and depend on the growth conditions.
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000052955 650_2 $$2MeSH$$aAnti-Bacterial Agents: pharmacology
000052955 650_2 $$2MeSH$$aCorynebacterium glutamicum: chemistry
000052955 650_2 $$2MeSH$$aCorynebacterium glutamicum: drug effects
000052955 650_2 $$2MeSH$$aCorynebacterium glutamicum: metabolism
000052955 650_2 $$2MeSH$$aEthambutol: pharmacology
000052955 650_2 $$2MeSH$$aOsmotic Pressure
000052955 650_2 $$2MeSH$$aPolyphosphates: analysis
000052955 650_2 $$2MeSH$$aPolyphosphates: metabolism
000052955 650_2 $$2MeSH$$aPolysorbates
000052955 650_2 $$2MeSH$$aSolubility
000052955 650_7 $$00$$2NLM Chemicals$$aAnti-Bacterial Agents
000052955 650_7 $$00$$2NLM Chemicals$$aPolyphosphates
000052955 650_7 $$00$$2NLM Chemicals$$aPolysorbates
000052955 650_7 $$074-55-5$$2NLM Chemicals$$aEthambutol
000052955 650_7 $$09005-66-7$$2NLM Chemicals$$apolysorbate 40
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000052955 65320 $$2Author$$apoly P
000052955 65320 $$2Author$$avolutin granules
000052955 65320 $$2Author$$aDAPI
000052955 65320 $$2Author$$aP-31 NMR
000052955 65320 $$2Author$$aphosphate starvation
000052955 65320 $$2Author$$aosmoshock
000052955 65320 $$2Author$$aethambutol
000052955 65320 $$2Author$$atween 40
000052955 65320 $$2Author$$aglutamate production
000052955 65320 $$2Author$$adigital image analysis
000052955 65320 $$2Author$$aCorynebacterium glutamicum
000052955 7001_ $$0P:(DE-HGF)0$$aPallerla, S. R.$$b1
000052955 7001_ $$0P:(DE-HGF)0$$aVidaurre, D.$$b2
000052955 7001_ $$0P:(DE-Juel1)VDB36624$$aRalfs, C.$$b3$$uFZJ
000052955 7001_ $$0P:(DE-HGF)0$$aWendisch, V. F.$$b4
000052955 7001_ $$0P:(DE-HGF)0$$aSchoberth, S. M.$$b5
000052955 773__ $$0PERI:(DE-600)1464336-4$$a10.1007/s00253-006-0562-8$$gVol. 72, p. 1099 - 1106$$p1099 - 1106$$q72<1099 - 1106$$tApplied Microbiology and Biotechnology$$v72$$x0175-7598$$y2006
000052955 8567_ $$uhttp://dx.doi.org/10.1007/s00253-006-0562-8
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