Journal Article

PreJuSER-52955

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Determination of soluble and granular inorganic polyphosphate in Corynebacterium glutamicum

Klauth, P.FZJ* ; Pallerla, S. R. ; Vidaurre, D. ; Ralfs, C.FZJ* ; Wendisch, V. F. ; Schoberth, S. M.

2006
Springer Berlin

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Please use a persistent id in citations: doi:10.1007/s00253-006-0562-8

Abstract: Corynebacterium glutamicum forms inorganic polyphosphate (poly P) that may occur as soluble (cytosolic) poly P and/or as volutin granules. A suitable method for monitoring soluble and granular poly P in C. glutamicum was developed and applied to C. glutamicum cells cultivated under different growth conditions. Under phosphate-limiting conditions, C. glutamicum did not accumulate poly P, but it rebuilt its poly P storages when phosphate became available. The poly P content of C. glutamicum growing on glucose minimal medium with sufficient phosphate varied considerably during growth. While the poly P content was minimal in the midexponential growth phase, two maxima were observed in the early exponential growth phase and at entry into the stationary growth phase. Cells in the early exponential growth phase primarily contained granular poly P, while cells entering the stationary growth phase contained soluble, cytosolic poly P. These results and those obtained for C. glutamicum cells cultivated under hypo- or hyperosmotic conditions or during glutamate production revealed that the poly P content of C. glutamicum and the partitioning between cytosolic and granular forms of poly P are dynamics and depend on the growth conditions.

Keyword(s): Anti-Bacterial Agents: pharmacology (MeSH) ; Corynebacterium glutamicum: chemistry (MeSH) ; Corynebacterium glutamicum: drug effects (MeSH) ; Corynebacterium glutamicum: metabolism (MeSH) ; Ethambutol: pharmacology (MeSH) ; Osmotic Pressure (MeSH) ; Polyphosphates: analysis (MeSH) ; Polyphosphates: metabolism (MeSH) ; Polysorbates (MeSH) ; Solubility (MeSH) ; Anti-Bacterial Agents ; Polyphosphates ; Polysorbates ; Ethambutol ; polysorbate 40 ; J ; poly P (auto) ; volutin granules (auto) ; DAPI (auto) ; P-31 NMR (auto) ; phosphate starvation (auto) ; osmoshock (auto) ; ethambutol (auto) ; tween 40 (auto) ; glutamate production (auto) ; digital image analysis (auto) ; Corynebacterium glutamicum (auto)

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Agrosphäre (ICG-IV)
2. Biotechnologie 1 (IBT-1)

Research Program(s):

1. Terrestrische Umwelt (P24)
2. Biotechnologie (PBT)

Appears in the scientific report 2006

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Database coverage:
; JCR ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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