TY  - JOUR
AU  - Klauth, P.
AU  - Pallerla, S. R.
AU  - Vidaurre, D.
AU  - Ralfs, C.
AU  - Wendisch, V. F.
AU  - Schoberth, S. M.
TI  - Determination of soluble and granular inorganic  polyphosphate in Corynebacterium glutamicum
JO  - Applied Microbiology and Biotechnology
VL  - 72
SN  - 0175-7598
CY  - Berlin
PB  - Springer
M1  - PreJuSER-52955
SP  - 1099 - 1106
PY  - 2006
N1  - Record converted from VDB: 12.11.2012
AB  - Corynebacterium glutamicum forms inorganic polyphosphate (poly P) that may occur as soluble (cytosolic) poly P and/or as volutin granules. A suitable method for monitoring soluble and granular poly P in C. glutamicum was developed and applied to C. glutamicum cells cultivated under different growth conditions. Under phosphate-limiting conditions, C. glutamicum did not accumulate poly P, but it rebuilt its poly P storages when phosphate became available. The poly P content of C. glutamicum growing on glucose minimal medium with sufficient phosphate varied considerably during growth. While the poly P content was minimal in the midexponential growth phase, two maxima were observed in the early exponential growth phase and at entry into the stationary growth phase. Cells in the early exponential growth phase primarily contained granular poly P, while cells entering the stationary growth phase contained soluble, cytosolic poly P. These results and those obtained for C. glutamicum cells cultivated under hypo- or hyperosmotic conditions or during glutamate production revealed that the poly P content of C. glutamicum and the partitioning between cytosolic and granular forms of poly P are dynamics and depend on the growth conditions.
KW  - Anti-Bacterial Agents: pharmacology
KW  - Corynebacterium glutamicum: chemistry
KW  - Corynebacterium glutamicum: drug effects
KW  - Corynebacterium glutamicum: metabolism
KW  - Ethambutol: pharmacology
KW  - Osmotic Pressure
KW  - Polyphosphates: analysis
KW  - Polyphosphates: metabolism
KW  - Polysorbates
KW  - Solubility
KW  - Anti-Bacterial Agents (NLM Chemicals)
KW  - Polyphosphates (NLM Chemicals)
KW  - Polysorbates (NLM Chemicals)
KW  - Ethambutol (NLM Chemicals)
KW  - polysorbate 40 (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:16977467
UR  - <Go to ISI:>//WOS:000240857300031
DO  - DOI:10.1007/s00253-006-0562-8
UR  - https://juser.fz-juelich.de/record/52955
ER  -