TY  - JOUR
AU  - Oszlanczi, E.
AU  - Bota, A.
AU  - Klumpp, E.
TI  - Layer formations in the bacteria-membrane mimetic DPPE-DPPG/water system induced by sulfadiazine
JO  - Biophysical chemistry
VL  - 125
SN  - 0301-4622
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - PreJuSER-55820
SP  - 334 - 340
PY  - 2007
N1  - Record converted from VDB: 12.11.2012
AB  - The effect of the frequently used antibiotic sulfadiazine (SD) was studied on a bacteria membrane mimetic model system by using differential scanning calorimetric (DSC), small- and wide-angle X-ray scattering (SWAXS) and freeze-fracture methods. The membrane model system consisted of dipalmitoylphosphatidylethanolamine (DPPE, 0.8 molar ratio) and dipalmitoylphosphatidylglycerol (DPPG, 0.2 molar ratio). The SD molar ratio (relative to the lipids) was varied between 10(-3) and 1. In the presence of SD, two transitions between the gel and liquid crystalline phases appear at 60.5 degrees C and about at 65 degrees C. In the temperature domain of the gel phase, the subcell of the chain packing is strongly temperature dependent indicating the increased dominance of the hydration forces during the first transition and the location of SD molecules in the neighbourhood of the polar lipid head groups. The second transition is accompanied by the changes in the nanometer-scale layer arrangements observed by SAXS and in the mum-scale morphology observed by freeze-fracture. Above the temperature of the second transition, the SD-induced metastable structures undergo further formations to produce a more homogeneous state favoured by the geometrical packing of the cylindrical-shaped lipid molecules.
KW  - Bacteria: ultrastructure
KW  - Calorimetry, Differential Scanning
KW  - Cell Membrane: chemistry
KW  - Freeze Fracturing
KW  - Models, Biological
KW  - Phase Transition
KW  - Phosphatidylethanolamines
KW  - Phosphatidylglycerols
KW  - Sulfadiazine
KW  - Temperature
KW  - Water
KW  - X-Ray Diffraction
KW  - Phosphatidylethanolamines (NLM Chemicals)
KW  - Phosphatidylglycerols (NLM Chemicals)
KW  - 1,2-dipalmitoyl-3-phosphatidylethanolamine (NLM Chemicals)
KW  - 1,2-dipalmitoylphosphatidylglycerol (NLM Chemicals)
KW  - Sulfadiazine (NLM Chemicals)
KW  - Water (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:17046146
UR  - <Go to ISI:>//WOS:000244219500013
DO  - DOI:10.1016/j.bpc.2006.09.008
UR  - https://juser.fz-juelich.de/record/55820
ER  -