Journal Article

PreJuSER-55820

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Layer formations in the bacteria-membrane mimetic DPPE-DPPG/water system induced by sulfadiazine

Oszlanczi, E. ; Bota, A. ; Klumpp, E.FZJ*

2007
Elsevier Science Amsterdam [u.a.]

This record in other databases:    

Please use a persistent id in citations: doi:10.1016/j.bpc.2006.09.008

Abstract: The effect of the frequently used antibiotic sulfadiazine (SD) was studied on a bacteria membrane mimetic model system by using differential scanning calorimetric (DSC), small- and wide-angle X-ray scattering (SWAXS) and freeze-fracture methods. The membrane model system consisted of dipalmitoylphosphatidylethanolamine (DPPE, 0.8 molar ratio) and dipalmitoylphosphatidylglycerol (DPPG, 0.2 molar ratio). The SD molar ratio (relative to the lipids) was varied between 10(-3) and 1. In the presence of SD, two transitions between the gel and liquid crystalline phases appear at 60.5 degrees C and about at 65 degrees C. In the temperature domain of the gel phase, the subcell of the chain packing is strongly temperature dependent indicating the increased dominance of the hydration forces during the first transition and the location of SD molecules in the neighbourhood of the polar lipid head groups. The second transition is accompanied by the changes in the nanometer-scale layer arrangements observed by SAXS and in the mum-scale morphology observed by freeze-fracture. Above the temperature of the second transition, the SD-induced metastable structures undergo further formations to produce a more homogeneous state favoured by the geometrical packing of the cylindrical-shaped lipid molecules.

Keyword(s): Bacteria: ultrastructure (MeSH) ; Calorimetry, Differential Scanning (MeSH) ; Cell Membrane: chemistry (MeSH) ; Freeze Fracturing (MeSH) ; Models, Biological (MeSH) ; Phase Transition (MeSH) ; Phosphatidylethanolamines (MeSH) ; Phosphatidylglycerols (MeSH) ; Sulfadiazine (MeSH) ; Temperature (MeSH) ; Water (MeSH) ; X-Ray Diffraction (MeSH) ; Phosphatidylethanolamines ; Phosphatidylglycerols ; 1,2-dipalmitoyl-3-phosphatidylethanolamine ; 1,2-dipalmitoylphosphatidylglycerol ; Sulfadiazine ; Water ; J ; sulfadiazine (auto) ; DPPE-DPPG vesicles (auto) ; phase separation (auto) ; DSC (auto) ; small- and wide-angle X-ray scattering (SWAXS) (auto) ; freeze-fracture (auto)

Note: Record converted from VDB: 12.11.2012

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Research Program(s):

1. Terrestrische Umwelt (P24)

Appears in the scientific report 2007

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