TY  - JOUR
AU  - Sengupta, K.
AU  - Aranda-Espinoza, H.
AU  - Smith, L.
AU  - Janmey, P.
AU  - Hammer, D.
TI  - Spreading of neutrophils: from activation to migration
JO  - Biophysical journal
VL  - 91
SN  - 0006-3495
CY  - New York, NY
PB  - Rockefeller Univ. Press
M1  - PreJuSER-57086
SP  - 4638 - 4648
PY  - 2006
N1  - Record converted from VDB: 12.11.2012
AB  - Neutrophils rely on rapid changes in morphology to ward off invaders. Time-resolved dynamics of spreading human neutrophils after activation by the chemoattractant fMLF (formyl methionyl leucyl phenylalanine) was observed by RICM (reflection interference contrast microscopy). An image-processing algorithm was developed to identify the changes in the overall cell shape and the zones of close contact with the substrate. We show that in the case of neutrophils, cell spreading immediately after exposure of fMLF is anisotropic and directional. The dependence of spreading area, A, of the cell as a function of time, t, shows several distinct regimes, each of which can be fitted as power laws (A ~ t(b)). The different spreading regimes correspond to distinct values of the exponent b and are related to the adhesion state of the cell. Treatment with cytochalasin-B eliminated the anisotropy in the spreading.
KW  - Actins: metabolism
KW  - Bicyclo Compounds, Heterocyclic: pharmacology
KW  - Cell Adhesion
KW  - Cell Polarity
KW  - Cell Shape
KW  - Chemotaxis, Leukocyte
KW  - Cytochalasin B: pharmacology
KW  - Humans
KW  - Models, Biological
KW  - N-Formylmethionine Leucyl-Phenylalanine: pharmacology
KW  - Neutrophil Activation
KW  - Neutrophils: physiology
KW  - Thiazolidines: pharmacology
KW  - Actins (NLM Chemicals)
KW  - Bicyclo Compounds, Heterocyclic (NLM Chemicals)
KW  - Thiazolidines (NLM Chemicals)
KW  - Cytochalasin B (NLM Chemicals)
KW  - N-Formylmethionine Leucyl-Phenylalanine (NLM Chemicals)
KW  - latrunculin A (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:17012330
C2  - pmc:PMC1779913
UR  - <Go to ISI:>//WOS:000242339600032
DO  - DOI:10.1529/biophysj.105.080382
UR  - https://juser.fz-juelich.de/record/57086
ER  -