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| Home > Publications database > Spreading of neutrophils: from activation to migration |
| Journal Article | PreJuSER-57086 |
; ; ; ;
2006
Rockefeller Univ. Press
New York, NY
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Please use a persistent id in citations: doi:10.1529/biophysj.105.080382
Abstract: Neutrophils rely on rapid changes in morphology to ward off invaders. Time-resolved dynamics of spreading human neutrophils after activation by the chemoattractant fMLF (formyl methionyl leucyl phenylalanine) was observed by RICM (reflection interference contrast microscopy). An image-processing algorithm was developed to identify the changes in the overall cell shape and the zones of close contact with the substrate. We show that in the case of neutrophils, cell spreading immediately after exposure of fMLF is anisotropic and directional. The dependence of spreading area, A, of the cell as a function of time, t, shows several distinct regimes, each of which can be fitted as power laws (A ~ t(b)). The different spreading regimes correspond to distinct values of the exponent b and are related to the adhesion state of the cell. Treatment with cytochalasin-B eliminated the anisotropy in the spreading.
Keyword(s): Actins: metabolism (MeSH) ; Bicyclo Compounds, Heterocyclic: pharmacology (MeSH) ; Cell Adhesion (MeSH) ; Cell Polarity (MeSH) ; Cell Shape (MeSH) ; Chemotaxis, Leukocyte (MeSH) ; Cytochalasin B: pharmacology (MeSH) ; Humans (MeSH) ; Models, Biological (MeSH) ; N-Formylmethionine Leucyl-Phenylalanine: pharmacology (MeSH) ; Neutrophil Activation (MeSH) ; Neutrophils: physiology (MeSH) ; Thiazolidines: pharmacology (MeSH) ; Actins ; Bicyclo Compounds, Heterocyclic ; Thiazolidines ; Cytochalasin B ; N-Formylmethionine Leucyl-Phenylalanine ; latrunculin A ; J
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