TY  - JOUR
AU  - Meyer, M. H. F.
AU  - Hartmann, M.
AU  - Krause, H.-J.
AU  - Blankenstein, G.
AU  - Müller-Chorus, B.
AU  - Oster, J.
AU  - Miethe, P.
AU  - Keusgen, M.
TI  - CRP determination based on a novel magnetic biosensor
JO  - Biosensors and bioelectronics
VL  - 22
SN  - 0956-5663
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - PreJuSER-57202
SP  - 973 - 979
PY  - 2007
N1  - Record converted from VDB: 12.11.2012
AB  - The c-reactive protein (CRP) is a very significant human blood marker for inflammatory processes and is routinely determined for many clinical purposes. The widespread and well established detection method for this approximately 115 kDa hepatic protein is the high-sensitivity ELISA assay (hsCRP-ELISA) in blood serum. New approaches in medical CRP diagnosis (e.g. for CVD, inflammatory bowel disease) require rapid quantification in native matrices. A novel CRP determination method based on magnetic detection is described and tested for human blood serum, saliva and urine. The detection principle is based on two different anti-CRP antibodies (monoclonal, IgG) for CRP trapment and labelling. The linear detection range of this immunosensor ranged from 25 ng/ml to 2.5 microg/ml and is therefore much more sensitive than typical hsCRP-ELISA-assays.
KW  - Biological Markers: blood
KW  - Biosensing Techniques: instrumentation
KW  - Biosensing Techniques: methods
KW  - Blood Chemical Analysis: instrumentation
KW  - Blood Chemical Analysis: methods
KW  - C-Reactive Protein: analysis
KW  - Equipment Design
KW  - Equipment Failure Analysis
KW  - Humans
KW  - Immunoassay: instrumentation
KW  - Immunoassay: methods
KW  - Immunomagnetic Separation: instrumentation
KW  - Immunomagnetic Separation: methods
KW  - Inflammation: blood
KW  - Inflammation: diagnosis
KW  - Magnetics: diagnostic use
KW  - Magnetics: instrumentation
KW  - Microchemistry: instrumentation
KW  - Microchemistry: methods
KW  - Sensitivity and Specificity
KW  - Biological Markers (NLM Chemicals)
KW  - C-Reactive Protein (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:16766177
UR  - <Go to ISI:>//WOS:000243541800027
DO  - DOI:10.1016/j.bios.2006.04.001
UR  - https://juser.fz-juelich.de/record/57202
ER  -