Journal Article

PreJuSER-57202

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png CRP determination based on a novel magnetic biosensor

Meyer, M. H. F. ; Hartmann, M. ; Krause, H.-J.FZJ* ; Blankenstein, G. ; Müller-Chorus, B. ; Oster, J. ; Miethe, P. ; Keusgen, M.FZJ*

2007
Elsevier Science Amsterdam [u.a.]

This record in other databases:      

Please use a persistent id in citations: doi:10.1016/j.bios.2006.04.001

Abstract: The c-reactive protein (CRP) is a very significant human blood marker for inflammatory processes and is routinely determined for many clinical purposes. The widespread and well established detection method for this approximately 115 kDa hepatic protein is the high-sensitivity ELISA assay (hsCRP-ELISA) in blood serum. New approaches in medical CRP diagnosis (e.g. for CVD, inflammatory bowel disease) require rapid quantification in native matrices. A novel CRP determination method based on magnetic detection is described and tested for human blood serum, saliva and urine. The detection principle is based on two different anti-CRP antibodies (monoclonal, IgG) for CRP trapment and labelling. The linear detection range of this immunosensor ranged from 25 ng/ml to 2.5 microg/ml and is therefore much more sensitive than typical hsCRP-ELISA-assays.

Keyword(s): Biological Markers: blood (MeSH) ; Biosensing Techniques: instrumentation (MeSH) ; Biosensing Techniques: methods (MeSH) ; Blood Chemical Analysis: instrumentation (MeSH) ; Blood Chemical Analysis: methods (MeSH) ; C-Reactive Protein: analysis (MeSH) ; Equipment Design (MeSH) ; Equipment Failure Analysis (MeSH) ; Humans (MeSH) ; Immunoassay: instrumentation (MeSH) ; Immunoassay: methods (MeSH) ; Immunomagnetic Separation: instrumentation (MeSH) ; Immunomagnetic Separation: methods (MeSH) ; Inflammation: blood (MeSH) ; Inflammation: diagnosis (MeSH) ; Magnetics: diagnostic use (MeSH) ; Magnetics: instrumentation (MeSH) ; Microchemistry: instrumentation (MeSH) ; Microchemistry: methods (MeSH) ; Sensitivity and Specificity (MeSH) ; Biological Markers ; C-Reactive Protein ; J ; c-reactive protein (CRP) (auto) ; magnetic detection (auto) ; immumosensor (auto) ; inflammation (auto) ; CVD (auto) ; IBD (auto)

Note: Record converted from VDB: 12.11.2012

---

Contributing Institute(s):

1. Bioelektronik (IBN-2)
2. Center of Nanoelectronic Systems for Information Technology (CNI)

Research Program(s):

1. Grundlagen für zukünftige Informationstechnologien (P42)

Appears in the scientific report 2007

---