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@ARTICLE{Meyer:57202,
author = {Meyer, M. H. F. and Hartmann, M. and Krause, H.-J. and
Blankenstein, G. and Müller-Chorus, B. and Oster, J. and
Miethe, P. and Keusgen, M.},
title = {{CRP} determination based on a novel magnetic biosensor},
journal = {Biosensors and bioelectronics},
volume = {22},
issn = {0956-5663},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {PreJuSER-57202},
pages = {973 - 979},
year = {2007},
note = {Record converted from VDB: 12.11.2012},
abstract = {The c-reactive protein (CRP) is a very significant human
blood marker for inflammatory processes and is routinely
determined for many clinical purposes. The widespread and
well established detection method for this approximately 115
kDa hepatic protein is the high-sensitivity ELISA assay
(hsCRP-ELISA) in blood serum. New approaches in medical CRP
diagnosis (e.g. for CVD, inflammatory bowel disease) require
rapid quantification in native matrices. A novel CRP
determination method based on magnetic detection is
described and tested for human blood serum, saliva and
urine. The detection principle is based on two different
anti-CRP antibodies (monoclonal, IgG) for CRP trapment and
labelling. The linear detection range of this immunosensor
ranged from 25 ng/ml to 2.5 microg/ml and is therefore much
more sensitive than typical hsCRP-ELISA-assays.},
keywords = {Biological Markers: blood / Biosensing Techniques:
instrumentation / Biosensing Techniques: methods / Blood
Chemical Analysis: instrumentation / Blood Chemical
Analysis: methods / C-Reactive Protein: analysis / Equipment
Design / Equipment Failure Analysis / Humans / Immunoassay:
instrumentation / Immunoassay: methods / Immunomagnetic
Separation: instrumentation / Immunomagnetic Separation:
methods / Inflammation: blood / Inflammation: diagnosis /
Magnetics: diagnostic use / Magnetics: instrumentation /
Microchemistry: instrumentation / Microchemistry: methods /
Sensitivity and Specificity / Biological Markers (NLM
Chemicals) / C-Reactive Protein (NLM Chemicals) / J
(WoSType)},
cin = {CNI / IBN-2},
ddc = {570},
cid = {I:(DE-Juel1)VDB381 / I:(DE-Juel1)IBN-2-20090406},
pnm = {Grundlagen für zukünftige Informationstechnologien},
pid = {G:(DE-Juel1)FUEK412},
shelfmark = {Biophysics / Biotechnology $\&$ Applied Microbiology /
Chemistry, Analytical / Electrochemistry / Nanoscience $\&$
Nanotechnology},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:16766177},
UT = {WOS:000243541800027},
doi = {10.1016/j.bios.2006.04.001},
url = {https://juser.fz-juelich.de/record/57202},
}