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000058310 0247_ $$2DOI$$a10.1016/j.bios.2006.11.019
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000058310 041__ $$aeng
000058310 082__ $$a570
000058310 084__ $$2WoS$$aBiophysics
000058310 084__ $$2WoS$$aBiotechnology & Applied Microbiology
000058310 084__ $$2WoS$$aChemistry, Analytical
000058310 084__ $$2WoS$$aElectrochemistry
000058310 084__ $$2WoS$$aNanoscience & Nanotechnology
000058310 1001_ $$0P:(DE-Juel1)VDB5728$$aIngebrandt, S.$$b0$$uFZJ
000058310 245__ $$aLabel-free detection of single nucleotide polymorphisms utilizing the differential transfer function
000058310 260__ $$aAmsterdam [u.a.]$$bElsevier Science$$c2007
000058310 300__ $$a2834 - 2840
000058310 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
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000058310 440_0 $$024721$$aBiosensors & Bioelectronics$$v22
000058310 500__ $$aRecord converted from VDB: 12.11.2012
000058310 520__ $$aWe present a label-free method for the detection of DNA hybridization, which is monitored by non-metallized silicon field-effect transistors (FET) in a microarray approach. The described method enables a fast and fully electronic readout of ex situ binding assays. The label-free detection utilizing the field-effect is based on the intrinsic charge of the DNA molecules and/or on changes of the solid-liquid interface impedance, when biomolecules bind to the sensor surface. With our sensor system, usually a time-resolved, dc readout is used. In general, this FET signal suffers from sensor drift, temperature drift, changes in electrolyte composition or pH value, influence of the reference electrode, etc. In this article, we present a differential ac readout concept for FET microarrays, which enables a stable operation of the sensor against many of these side-parameters, reliable readout and a possibility for a quick screening of large sensor arrays. We present the detection of point mutations in short DNA samples with this method in an ex situ binding assay.
000058310 536__ $$0G:(DE-Juel1)FUEK412$$2G:(DE-HGF)$$aGrundlagen für zukünftige Informationstechnologien$$cP42$$x0
000058310 588__ $$aDataset connected to Web of Science, Pubmed
000058310 650_2 $$2MeSH$$aBiosensing Techniques: instrumentation
000058310 650_2 $$2MeSH$$aBiosensing Techniques: methods
000058310 650_2 $$2MeSH$$aDNA: analysis
000058310 650_2 $$2MeSH$$aDNA: genetics
000058310 650_2 $$2MeSH$$aNucleic Acid Hybridization
000058310 650_2 $$2MeSH$$aPolymorphism, Single Nucleotide
000058310 650_2 $$2MeSH$$aPotentiometry
000058310 650_2 $$2MeSH$$aTransistors, Electronic
000058310 650_7 $$09007-49-2$$2NLM Chemicals$$aDNA
000058310 650_7 $$2WoSType$$aJ
000058310 65320 $$2Author$$aDNA
000058310 65320 $$2Author$$alabel-free detection
000058310 65320 $$2Author$$afield-effect transistor
000058310 65320 $$2Author$$atransfer function
000058310 65320 $$2Author$$aimpedance spectroscopy
000058310 65320 $$2Author$$asingle nucleotide polymorphism
000058310 7001_ $$0P:(DE-Juel1)VDB34017$$aHan, Y.$$b1$$uFZJ
000058310 7001_ $$0P:(DE-HGF)0$$aNakamura, F.$$b2
000058310 7001_ $$0P:(DE-Juel1)VDB1267$$aPoghossian, A.$$b3$$uFZJ
000058310 7001_ $$0P:(DE-Juel1)128727$$aSchöning, M. J.$$b4$$uFZJ
000058310 7001_ $$0P:(DE-Juel1)128713$$aOffenhäusser, A.$$b5$$uFZJ
000058310 773__ $$0PERI:(DE-600)1496379-6$$a10.1016/j.bios.2006.11.019$$gVol. 22, p. 2834 - 2840$$p2834 - 2840$$q22<2834 - 2840$$tBiosensors and bioelectronics$$v22$$x0956-5663$$y2007
000058310 8567_ $$uhttp://dx.doi.org/10.1016/j.bios.2006.11.019
000058310 909CO $$ooai:juser.fz-juelich.de:58310$$pVDB
000058310 9131_ $$0G:(DE-Juel1)FUEK412$$bSchlüsseltechnologien$$kP42$$lGrundlagen für zukünftige Informationstechnologien (FIT)$$vGrundlagen für zukünftige Informationstechnologien$$x0
000058310 9141_ $$y2007
000058310 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000058310 9201_ $$0I:(DE-Juel1)VDB381$$d14.09.2008$$gCNI$$kCNI$$lCenter of Nanoelectronic Systems for Information Technology$$x1$$z381
000058310 9201_ $$0I:(DE-Juel1)IBN-2-20090406$$d31.12.2010$$gIBN$$kIBN-2$$lBioelektronik$$x0
000058310 9201_ $$0I:(DE-82)080009_20140620$$gJARA$$kJARA-FIT$$lJülich-Aachen Research Alliance - Fundamentals of Future Information Technology$$x2
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