Journal Article

PreJuSER-58310

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Label-free detection of single nucleotide polymorphisms utilizing the differential transfer function

Ingebrandt, S.FZJ* ; Han, Y.FZJ* ; Nakamura, F. ; Poghossian, A.FZJ* ; Schöning, M. J.FZJ* ; Offenhäusser, A.FZJ*

2007
Elsevier Science Amsterdam [u.a.]

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Please use a persistent id in citations: doi:10.1016/j.bios.2006.11.019

Abstract: We present a label-free method for the detection of DNA hybridization, which is monitored by non-metallized silicon field-effect transistors (FET) in a microarray approach. The described method enables a fast and fully electronic readout of ex situ binding assays. The label-free detection utilizing the field-effect is based on the intrinsic charge of the DNA molecules and/or on changes of the solid-liquid interface impedance, when biomolecules bind to the sensor surface. With our sensor system, usually a time-resolved, dc readout is used. In general, this FET signal suffers from sensor drift, temperature drift, changes in electrolyte composition or pH value, influence of the reference electrode, etc. In this article, we present a differential ac readout concept for FET microarrays, which enables a stable operation of the sensor against many of these side-parameters, reliable readout and a possibility for a quick screening of large sensor arrays. We present the detection of point mutations in short DNA samples with this method in an ex situ binding assay.

Keyword(s): Biosensing Techniques: instrumentation (MeSH) ; Biosensing Techniques: methods (MeSH) ; DNA: analysis (MeSH) ; DNA: genetics (MeSH) ; Nucleic Acid Hybridization (MeSH) ; Polymorphism, Single Nucleotide (MeSH) ; Potentiometry (MeSH) ; Transistors, Electronic (MeSH) ; DNA ; J ; DNA (auto) ; label-free detection (auto) ; field-effect transistor (auto) ; transfer function (auto) ; impedance spectroscopy (auto) ; single nucleotide polymorphism (auto)

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Bioelektronik (IBN-2)
2. Center of Nanoelectronic Systems for Information Technology (CNI)
3. Jülich-Aachen Research Alliance - Fundamentals of Future Information Technology (JARA-FIT)

Research Program(s):

1. Grundlagen für zukünftige Informationstechnologien (P42)

Appears in the scientific report 2007

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