000058487 001__ 58487 000058487 005__ 20200402210414.0 000058487 0247_ $$2pmid$$apmid:17660320 000058487 0247_ $$2pmc$$apmc:PMC2025665 000058487 0247_ $$2DOI$$a10.1529/biophysj.107.111328 000058487 0247_ $$2WOS$$aWOS:000250199300036 000058487 037__ $$aPreJuSER-58487 000058487 041__ $$aeng 000058487 082__ $$a570 000058487 084__ $$2WoS$$aBiophysics 000058487 1001_ $$0P:(DE-Juel1)128833$$aMerkel, R.$$b0$$uFZJ 000058487 245__ $$aCell Force Microscopy on Elastic Layers of Finite Thickness 000058487 260__ $$aNew York, NY$$bRockefeller Univ. Press$$c2007 000058487 300__ $$a3314 - 3323 000058487 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article 000058487 3367_ $$2DataCite$$aOutput Types/Journal article 000058487 3367_ $$00$$2EndNote$$aJournal Article 000058487 3367_ $$2BibTeX$$aARTICLE 000058487 3367_ $$2ORCID$$aJOURNAL_ARTICLE 000058487 3367_ $$2DRIVER$$aarticle 000058487 440_0 $$0882$$aBiophysical Journal$$v93$$x0006-3495 000058487 500__ $$aRecord converted from VDB: 12.11.2012 000058487 520__ $$aForces applied by cells to substrates can be measured using soft substrates with embedded displacement markers. Traction forces are retrieved from microscopic images by determining the displacements of these markers and fitting the generating forces. Here we show that using elastic films of 5-10-microm thickness one can improve the spatial resolution of the technique. To this end we derived explicit equations for the mechanical response of an elastic layer of finite thickness to point forces. Moreover, these equations allow highly accurate force measurements on eukaryotic cells on films where finite thickness effects are relevant (below approximately 60 microm). 000058487 536__ $$0G:(DE-Juel1)FUEK414$$2G:(DE-HGF)$$aKondensierte Materie$$cP54$$x0 000058487 588__ $$aDataset connected to Web of Science, Pubmed 000058487 650_2 $$2MeSH$$aAnimals 000058487 650_2 $$2MeSH$$aElasticity 000058487 650_2 $$2MeSH$$aFibroblasts: ultrastructure 000058487 650_2 $$2MeSH$$aMicroscopy, Atomic Force 000058487 650_2 $$2MeSH$$aMyocytes, Cardiac: ultrastructure 000058487 650_2 $$2MeSH$$aRats 000058487 650_2 $$2MeSH$$aRats, Wistar 000058487 650_2 $$2MeSH$$aSilicon 000058487 650_7 $$07440-21-3$$2NLM Chemicals$$aSilicon 000058487 650_7 $$2WoSType$$aJ 000058487 7001_ $$0P:(DE-Juel1)VDB8902$$aKirchgeßner, N.$$b1$$uFZJ 000058487 7001_ $$0P:(DE-Juel1)VDB57503$$aCesa, C. M.$$b2$$uFZJ 000058487 7001_ $$0P:(DE-Juel1)VDB27696$$aHoffmann, B.$$b3$$uFZJ 000058487 773__ $$0PERI:(DE-600)1477214-0$$a10.1529/biophysj.107.111328$$gVol. 93, p. 3314 - 3323$$p3314 - 3323$$q93<3314 - 3323$$tBiophysical journal$$v93$$x0006-3495$$y2007 000058487 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2025665 000058487 909CO $$ooai:juser.fz-juelich.de:58487$$pVDB 000058487 9131_ $$0G:(DE-Juel1)FUEK414$$bMaterie$$kP54$$lKondensierte Materie$$vKondensierte Materie$$x0$$zentfällt bis 2009 000058487 9141_ $$y2007 000058487 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed 000058487 9201_ $$0I:(DE-Juel1)VDB802$$d31.12.2010$$gIBN$$kIBN-4$$lBiomechanik$$x0 000058487 970__ $$aVDB:(DE-Juel1)92103 000058487 980__ $$aVDB 000058487 980__ $$aConvertedRecord 000058487 980__ $$ajournal 000058487 980__ $$aI:(DE-Juel1)ICS-7-20110106 000058487 980__ $$aUNRESTRICTED 000058487 981__ $$aI:(DE-Juel1)IBI-2-20200312 000058487 981__ $$aI:(DE-Juel1)ICS-7-20110106