Journal Article

PreJuSER-58487

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Cell Force Microscopy on Elastic Layers of Finite Thickness

Merkel, R.FZJ* ; Kirchgeßner, N.FZJ* ; Cesa, C. M.FZJ* ; Hoffmann, B.FZJ*

2007
Rockefeller Univ. Press New York, NY

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Please use a persistent id in citations: doi:10.1529/biophysj.107.111328

Abstract: Forces applied by cells to substrates can be measured using soft substrates with embedded displacement markers. Traction forces are retrieved from microscopic images by determining the displacements of these markers and fitting the generating forces. Here we show that using elastic films of 5-10-microm thickness one can improve the spatial resolution of the technique. To this end we derived explicit equations for the mechanical response of an elastic layer of finite thickness to point forces. Moreover, these equations allow highly accurate force measurements on eukaryotic cells on films where finite thickness effects are relevant (below approximately 60 microm).

Keyword(s): Animals (MeSH) ; Elasticity (MeSH) ; Fibroblasts: ultrastructure (MeSH) ; Microscopy, Atomic Force (MeSH) ; Myocytes, Cardiac: ultrastructure (MeSH) ; Rats (MeSH) ; Rats, Wistar (MeSH) ; Silicon (MeSH) ; Silicon ; J

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Biomechanik (IBN-4)

Research Program(s):

1. Kondensierte Materie (P54)

Appears in the scientific report 2007

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