TY  - JOUR
AU  - Merkel, R.
AU  - Kirchgeßner, N.
AU  - Cesa, C. M.
AU  - Hoffmann, B.
TI  - Cell Force Microscopy on Elastic Layers of Finite Thickness
JO  - Biophysical journal
VL  - 93
SN  - 0006-3495
CY  - New York, NY
PB  - Rockefeller Univ. Press
M1  - PreJuSER-58487
SP  - 3314 - 3323
PY  - 2007
N1  - Record converted from VDB: 12.11.2012
AB  - Forces applied by cells to substrates can be measured using soft substrates with embedded displacement markers. Traction forces are retrieved from microscopic images by determining the displacements of these markers and fitting the generating forces. Here we show that using elastic films of 5-10-microm thickness one can improve the spatial resolution of the technique. To this end we derived explicit equations for the mechanical response of an elastic layer of finite thickness to point forces. Moreover, these equations allow highly accurate force measurements on eukaryotic cells on films where finite thickness effects are relevant (below approximately 60 microm).
KW  - Animals
KW  - Elasticity
KW  - Fibroblasts: ultrastructure
KW  - Microscopy, Atomic Force
KW  - Myocytes, Cardiac: ultrastructure
KW  - Rats
KW  - Rats, Wistar
KW  - Silicon
KW  - Silicon (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:17660320
C2  - pmc:PMC2025665
UR  - <Go to ISI:>//WOS:000250199300036
DO  - DOI:10.1529/biophysj.107.111328
UR  - https://juser.fz-juelich.de/record/58487
ER  -