TY  - JOUR
AU  - Kohlmann, C.
AU  - Greiner, L.
AU  - Leitner, W.
AU  - Wandrey, C.
AU  - Lütz, S.
TI  - Ionic Liquides as Performance Additives for Electroenzymatic Syntheses
JO  - Chemistry
VL  - 15
SN  - 0947-6539
CY  - Weinheim
PB  - Wiley-VCH
M1  - PreJuSER-5895
SP  - 11692 - 11700
PY  - 2009
N1  - The authors thank Lilia Harter, Stephanie Corsten and Zubeyda Dogan for excellent work in the lab and the GK 1166 "BioNoCo" for fruitful discussion and funding. L.G. thanks the cluster of excellence "Tailor-Made Fuels from Biomass" (TMFB) for support.
AB  - Electroenzymatic syntheses combine oxidoreductase-catalysed reactions with electrochemical reactant supply. The use of ionic liquids as performance additives can contribute to overcoming existing limitations of these syntheses. Here, we report on the influence of different water-miscible ionic liquids on critical parameters such as conductivity, biocatalyst activity and stability or substrate solubility for three typical electroenzymatic syntheses. In these investigations promising ionic liquids were identified and have been used as additives for batch electrolyses on preparative scale for the three electroenzymatic systems. It was possible to improve the space-time-yield for the electrochemical regeneration of NADPH by a factor of three. For an amino acid oxidase catalysed resolution of a methionine racemate with ferrocene-mediated electrochemical regeneration of the enzyme-bound cofactor FAD a 50% increase in space time yield and 140% increase in catalyst utilisation (TTN) were achieved. Furthermore, for the chloroperoxidase-catalysed synthesis of (R)-phenylmethylsulfoxide with electrochemical generation of the required cosubstrate H2O2 the space time yield and the catalyst utilisation were improved by a factor of up to 4.2 depending on the ionic liquids used.
KW  - Amino Acid Oxidoreductases: metabolism
KW  - Biocatalysis
KW  - Chloride Peroxidase: metabolism
KW  - Electrochemical Techniques
KW  - Flavin-Adenine Dinucleotide: metabolism
KW  - Hydrogen Peroxide: metabolism
KW  - Ionic Liquids: chemistry
KW  - NADP: metabolism
KW  - Solubility
KW  - Ionic Liquids (NLM Chemicals)
KW  - Flavin-Adenine Dinucleotide (NLM Chemicals)
KW  - NADP (NLM Chemicals)
KW  - Hydrogen Peroxide (NLM Chemicals)
KW  - Chloride Peroxidase (NLM Chemicals)
KW  - Amino Acid Oxidoreductases (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:19777513
UR  - <Go to ISI:>//WOS:000271992100033
DO  - DOI:10.1002/chem.200901046
UR  - https://juser.fz-juelich.de/record/5895
ER  -