Journal Article

PreJuSER-5895

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Ionic Liquides as Performance Additives for Electroenzymatic Syntheses

Kohlmann, C.FZJ* ; Greiner, L. ; Leitner, W. ; Wandrey, C.FZJ* ; Lütz, S.FZJ*

2009
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/chem.200901046

Abstract: Electroenzymatic syntheses combine oxidoreductase-catalysed reactions with electrochemical reactant supply. The use of ionic liquids as performance additives can contribute to overcoming existing limitations of these syntheses. Here, we report on the influence of different water-miscible ionic liquids on critical parameters such as conductivity, biocatalyst activity and stability or substrate solubility for three typical electroenzymatic syntheses. In these investigations promising ionic liquids were identified and have been used as additives for batch electrolyses on preparative scale for the three electroenzymatic systems. It was possible to improve the space-time-yield for the electrochemical regeneration of NADPH by a factor of three. For an amino acid oxidase catalysed resolution of a methionine racemate with ferrocene-mediated electrochemical regeneration of the enzyme-bound cofactor FAD a 50% increase in space time yield and 140% increase in catalyst utilisation (TTN) were achieved. Furthermore, for the chloroperoxidase-catalysed synthesis of (R)-phenylmethylsulfoxide with electrochemical generation of the required cosubstrate H2O2 the space time yield and the catalyst utilisation were improved by a factor of up to 4.2 depending on the ionic liquids used.

Keyword(s): Amino Acid Oxidoreductases: metabolism (MeSH) ; Biocatalysis (MeSH) ; Chloride Peroxidase: metabolism (MeSH) ; Electrochemical Techniques (MeSH) ; Flavin-Adenine Dinucleotide: metabolism (MeSH) ; Hydrogen Peroxide: metabolism (MeSH) ; Ionic Liquids: chemistry (MeSH) ; NADP: metabolism (MeSH) ; Solubility (MeSH) ; Ionic Liquids ; Flavin-Adenine Dinucleotide ; NADP ; Hydrogen Peroxide ; Chloride Peroxidase ; Amino Acid Oxidoreductases ; J ; biocatalysis (auto) ; biotechnology (auto) ; electroenzymatic synthesis (auto) ; ionic liquids (auto) ; sustainable chemistry (auto)

Note: The authors thank Lilia Harter, Stephanie Corsten and Zubeyda Dogan for excellent work in the lab and the GK 1166 "BioNoCo" for fruitful discussion and funding. L.G. thanks the cluster of excellence "Tailor-Made Fuels from Biomass" (TMFB) for support.

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Contributing Institute(s):

1. Biotechnologie 2 (IBT-2)

Research Program(s):

1. Biotechnologie (PBT)

Appears in the scientific report 2009

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