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@ARTICLE{Kohlmann:5895,
author = {Kohlmann, C. and Greiner, L. and Leitner, W. and Wandrey,
C. and Lütz, S.},
title = {{I}onic {L}iquides as {P}erformance {A}dditives for
{E}lectroenzymatic {S}yntheses},
journal = {Chemistry},
volume = {15},
issn = {0947-6539},
address = {Weinheim},
publisher = {Wiley-VCH},
reportid = {PreJuSER-5895},
pages = {11692 - 11700},
year = {2009},
note = {The authors thank Lilia Harter, Stephanie Corsten and
Zubeyda Dogan for excellent work in the lab and the GK 1166
"BioNoCo" for fruitful discussion and funding. L.G. thanks
the cluster of excellence "Tailor-Made Fuels from Biomass"
(TMFB) for support.},
abstract = {Electroenzymatic syntheses combine oxidoreductase-catalysed
reactions with electrochemical reactant supply. The use of
ionic liquids as performance additives can contribute to
overcoming existing limitations of these syntheses. Here, we
report on the influence of different water-miscible ionic
liquids on critical parameters such as conductivity,
biocatalyst activity and stability or substrate solubility
for three typical electroenzymatic syntheses. In these
investigations promising ionic liquids were identified and
have been used as additives for batch electrolyses on
preparative scale for the three electroenzymatic systems. It
was possible to improve the space-time-yield for the
electrochemical regeneration of NADPH by a factor of three.
For an amino acid oxidase catalysed resolution of a
methionine racemate with ferrocene-mediated electrochemical
regeneration of the enzyme-bound cofactor FAD a $50\%$
increase in space time yield and $140\%$ increase in
catalyst utilisation (TTN) were achieved. Furthermore, for
the chloroperoxidase-catalysed synthesis of
(R)-phenylmethylsulfoxide with electrochemical generation of
the required cosubstrate H2O2 the space time yield and the
catalyst utilisation were improved by a factor of up to 4.2
depending on the ionic liquids used.},
keywords = {Amino Acid Oxidoreductases: metabolism / Biocatalysis /
Chloride Peroxidase: metabolism / Electrochemical Techniques
/ Flavin-Adenine Dinucleotide: metabolism / Hydrogen
Peroxide: metabolism / Ionic Liquids: chemistry / NADP:
metabolism / Solubility / Ionic Liquids (NLM Chemicals) /
Flavin-Adenine Dinucleotide (NLM Chemicals) / NADP (NLM
Chemicals) / Hydrogen Peroxide (NLM Chemicals) / Chloride
Peroxidase (NLM Chemicals) / Amino Acid Oxidoreductases (NLM
Chemicals) / J (WoSType)},
cin = {IBT-2},
ddc = {540},
cid = {I:(DE-Juel1)VDB56},
pnm = {Biotechnologie},
pid = {G:(DE-Juel1)FUEK410},
shelfmark = {Chemistry, Multidisciplinary},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:19777513},
UT = {WOS:000271992100033},
doi = {10.1002/chem.200901046},
url = {https://juser.fz-juelich.de/record/5895},
}