%0 Journal Article
%A Blombach, B.
%A Schreiner, M. E.
%A Holatko, J.
%A Bartek, T.
%A Oldiges, M.
%A Eikmanns, B. J.
%T L-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum
%J Applied and environmental microbiology
%V 73
%@ 0099-2240
%C Washington, DC [u.a.]
%I Soc.
%M PreJuSER-59150
%P 2079 - 2084
%D 2007
%Z Record converted from VDB: 12.11.2012
%X Corynebacterium glutamicum was engineered for the production of L-valine from glucose by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. In the absence of cellular growth, C. glutamicum DeltaaceE showed a relatively high intracellular concentration of pyruvate (25.9 mM) and produced significant amounts of pyruvate, L-alanine, and L-valine from glucose as the sole carbon source. Lactate or acetate was not formed. Plasmid-bound overexpression of ilvBNCE in C. glutamicum DeltaaceE resulted in an approximately 10-fold-lower intracellular pyruvate concentration (2.3 mM) and a shift of the extracellular product pattern from pyruvate and L-alanine towards L-valine. In fed-batch fermentations at high cell densities and an excess of glucose, C. glutamicum DeltaaceE(pJC4ilvBNCE) produced up to 210 mM L-valine with a volumetric productivity of 10.0 mM h(-1) (1.17 g l(-1) h(-1)) and a maximum yield of about 0.6 mol per mol (0.4 g per g) of glucose.
%K Alanine: biosynthesis
%K Corynebacterium glutamicum: metabolism
%K Fermentation
%K Isoleucine: biosynthesis
%K Lysine: biosynthesis
%K Pyruvate Dehydrogenase Complex: genetics
%K Pyruvate Dehydrogenase Complex: physiology
%K Pyruvic Acid: metabolism
%K Valine: biosynthesis
%K Pyruvate Dehydrogenase Complex (NLM Chemicals)
%K Pyruvic Acid (NLM Chemicals)
%K Alanine (NLM Chemicals)
%K Lysine (NLM Chemicals)
%K Valine (NLM Chemicals)
%K Isoleucine (NLM Chemicals)
%K J (WoSType)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:17293513
%2 pmc:PMC1855657
%U <Go to ISI:>//WOS:000245576300006
%R 10.1128/AEM.02826-06
%U https://juser.fz-juelich.de/record/59150