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| Home > Publications database > L-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum |
| Home > Publications database > L-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum |
| Journal Article | PreJuSER-59150 |
; ; ; ; ;
2007
Soc.
Washington, DC [u.a.]
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Please use a persistent id in citations: doi:10.1128/AEM.02826-06
Abstract: Corynebacterium glutamicum was engineered for the production of L-valine from glucose by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. In the absence of cellular growth, C. glutamicum DeltaaceE showed a relatively high intracellular concentration of pyruvate (25.9 mM) and produced significant amounts of pyruvate, L-alanine, and L-valine from glucose as the sole carbon source. Lactate or acetate was not formed. Plasmid-bound overexpression of ilvBNCE in C. glutamicum DeltaaceE resulted in an approximately 10-fold-lower intracellular pyruvate concentration (2.3 mM) and a shift of the extracellular product pattern from pyruvate and L-alanine towards L-valine. In fed-batch fermentations at high cell densities and an excess of glucose, C. glutamicum DeltaaceE(pJC4ilvBNCE) produced up to 210 mM L-valine with a volumetric productivity of 10.0 mM h(-1) (1.17 g l(-1) h(-1)) and a maximum yield of about 0.6 mol per mol (0.4 g per g) of glucose.
Keyword(s): Alanine: biosynthesis (MeSH) ; Corynebacterium glutamicum: metabolism (MeSH) ; Fermentation (MeSH) ; Isoleucine: biosynthesis (MeSH) ; Lysine: biosynthesis (MeSH) ; Pyruvate Dehydrogenase Complex: genetics (MeSH) ; Pyruvate Dehydrogenase Complex: physiology (MeSH) ; Pyruvic Acid: metabolism (MeSH) ; Valine: biosynthesis (MeSH) ; Pyruvate Dehydrogenase Complex ; Pyruvic Acid ; Alanine ; Lysine ; Valine ; Isoleucine ; J
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