000059150 001__ 59150
000059150 005__ 20190625111501.0
000059150 0247_ $$2pmid$$apmid:17293513
000059150 0247_ $$2pmc$$apmc:PMC1855657
000059150 0247_ $$2DOI$$a10.1128/AEM.02826-06
000059150 0247_ $$2WOS$$aWOS:000245576300006
000059150 0247_ $$2altmetric$$aaltmetric:21817993
000059150 037__ $$aPreJuSER-59150
000059150 041__ $$aeng
000059150 082__ $$a570
000059150 084__ $$2WoS$$aBiotechnology & Applied Microbiology
000059150 084__ $$2WoS$$aMicrobiology
000059150 1001_ $$0P:(DE-HGF)0$$aBlombach, B.$$b0
000059150 245__ $$aL-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum
000059150 260__ $$aWashington, DC [u.a.]$$bSoc.$$c2007
000059150 300__ $$a2079 - 2084
000059150 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article
000059150 3367_ $$2DataCite$$aOutput Types/Journal article
000059150 3367_ $$00$$2EndNote$$aJournal Article
000059150 3367_ $$2BibTeX$$aARTICLE
000059150 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000059150 3367_ $$2DRIVER$$aarticle
000059150 440_0 $$08561$$aApplied and Environmental Microbiology$$v73$$x0099-2240$$y7
000059150 500__ $$aRecord converted from VDB: 12.11.2012
000059150 520__ $$aCorynebacterium glutamicum was engineered for the production of L-valine from glucose by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. In the absence of cellular growth, C. glutamicum DeltaaceE showed a relatively high intracellular concentration of pyruvate (25.9 mM) and produced significant amounts of pyruvate, L-alanine, and L-valine from glucose as the sole carbon source. Lactate or acetate was not formed. Plasmid-bound overexpression of ilvBNCE in C. glutamicum DeltaaceE resulted in an approximately 10-fold-lower intracellular pyruvate concentration (2.3 mM) and a shift of the extracellular product pattern from pyruvate and L-alanine towards L-valine. In fed-batch fermentations at high cell densities and an excess of glucose, C. glutamicum DeltaaceE(pJC4ilvBNCE) produced up to 210 mM L-valine with a volumetric productivity of 10.0 mM h(-1) (1.17 g l(-1) h(-1)) and a maximum yield of about 0.6 mol per mol (0.4 g per g) of glucose.
000059150 536__ $$0G:(DE-Juel1)FUEK410$$2G:(DE-HGF)$$aBiotechnologie$$cPBT$$x0
000059150 588__ $$aDataset connected to Web of Science, Pubmed
000059150 650_2 $$2MeSH$$aAlanine: biosynthesis
000059150 650_2 $$2MeSH$$aCorynebacterium glutamicum: metabolism
000059150 650_2 $$2MeSH$$aFermentation
000059150 650_2 $$2MeSH$$aIsoleucine: biosynthesis
000059150 650_2 $$2MeSH$$aLysine: biosynthesis
000059150 650_2 $$2MeSH$$aPyruvate Dehydrogenase Complex: genetics
000059150 650_2 $$2MeSH$$aPyruvate Dehydrogenase Complex: physiology
000059150 650_2 $$2MeSH$$aPyruvic Acid: metabolism
000059150 650_2 $$2MeSH$$aValine: biosynthesis
000059150 650_7 $$00$$2NLM Chemicals$$aPyruvate Dehydrogenase Complex
000059150 650_7 $$0127-17-3$$2NLM Chemicals$$aPyruvic Acid
000059150 650_7 $$056-41-7$$2NLM Chemicals$$aAlanine
000059150 650_7 $$056-87-1$$2NLM Chemicals$$aLysine
000059150 650_7 $$07004-03-7$$2NLM Chemicals$$aValine
000059150 650_7 $$073-32-5$$2NLM Chemicals$$aIsoleucine
000059150 650_7 $$2WoSType$$aJ
000059150 7001_ $$0P:(DE-HGF)0$$aSchreiner, M. E.$$b1
000059150 7001_ $$0P:(DE-HGF)0$$aHolatko, J.$$b2
000059150 7001_ $$0P:(DE-Juel1)VDB59554$$aBartek, T.$$b3$$uFZJ
000059150 7001_ $$0P:(DE-Juel1)129053$$aOldiges, M.$$b4$$uFZJ
000059150 7001_ $$0P:(DE-HGF)0$$aEikmanns, B. J.$$b5
000059150 773__ $$0PERI:(DE-600)1478346-0$$a10.1128/AEM.02826-06$$gVol. 73, p. 2079 - 2084$$p2079 - 2084$$q73<2079 - 2084$$tApplied and environmental microbiology$$v73$$x0099-2240$$y2007
000059150 8567_ $$2Pubmed Central$$uhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC1855657
000059150 909CO $$ooai:juser.fz-juelich.de:59150$$pVDB
000059150 9131_ $$0G:(DE-Juel1)FUEK410$$baußerhalb PoF$$kPBT$$lohne FE$$vBiotechnologie$$x0
000059150 9141_ $$y2007
000059150 915__ $$0StatID:(DE-HGF)0010$$aJCR/ISI refereed
000059150 9201_ $$0I:(DE-Juel1)VDB56$$gIBT$$kIBT-2$$lBiotechnologie 2$$x1$$zab 31.10.10 weitergeführt IBG-1
000059150 970__ $$aVDB:(DE-Juel1)93111
000059150 980__ $$aVDB
000059150 980__ $$aConvertedRecord
000059150 980__ $$ajournal
000059150 980__ $$aI:(DE-Juel1)IBG-1-20101118
000059150 980__ $$aUNRESTRICTED
000059150 981__ $$aI:(DE-Juel1)IBG-1-20101118