TY  - JOUR
AU  - Blombach, B.
AU  - Schreiner, M. E.
AU  - Holatko, J.
AU  - Bartek, T.
AU  - Oldiges, M.
AU  - Eikmanns, B. J.
TI  - L-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum
JO  - Applied and environmental microbiology
VL  - 73
SN  - 0099-2240
CY  - Washington, DC [u.a.]
PB  - Soc.
M1  - PreJuSER-59150
SP  - 2079 - 2084
PY  - 2007
N1  - Record converted from VDB: 12.11.2012
AB  - Corynebacterium glutamicum was engineered for the production of L-valine from glucose by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. In the absence of cellular growth, C. glutamicum DeltaaceE showed a relatively high intracellular concentration of pyruvate (25.9 mM) and produced significant amounts of pyruvate, L-alanine, and L-valine from glucose as the sole carbon source. Lactate or acetate was not formed. Plasmid-bound overexpression of ilvBNCE in C. glutamicum DeltaaceE resulted in an approximately 10-fold-lower intracellular pyruvate concentration (2.3 mM) and a shift of the extracellular product pattern from pyruvate and L-alanine towards L-valine. In fed-batch fermentations at high cell densities and an excess of glucose, C. glutamicum DeltaaceE(pJC4ilvBNCE) produced up to 210 mM L-valine with a volumetric productivity of 10.0 mM h(-1) (1.17 g l(-1) h(-1)) and a maximum yield of about 0.6 mol per mol (0.4 g per g) of glucose.
KW  - Alanine: biosynthesis
KW  - Corynebacterium glutamicum: metabolism
KW  - Fermentation
KW  - Isoleucine: biosynthesis
KW  - Lysine: biosynthesis
KW  - Pyruvate Dehydrogenase Complex: genetics
KW  - Pyruvate Dehydrogenase Complex: physiology
KW  - Pyruvic Acid: metabolism
KW  - Valine: biosynthesis
KW  - Pyruvate Dehydrogenase Complex (NLM Chemicals)
KW  - Pyruvic Acid (NLM Chemicals)
KW  - Alanine (NLM Chemicals)
KW  - Lysine (NLM Chemicals)
KW  - Valine (NLM Chemicals)
KW  - Isoleucine (NLM Chemicals)
KW  - J (WoSType)
LB  - PUB:(DE-HGF)16
C6  - pmid:17293513
C2  - pmc:PMC1855657
UR  - <Go to ISI:>//WOS:000245576300006
DO  - DOI:10.1128/AEM.02826-06
UR  - https://juser.fz-juelich.de/record/59150
ER  -