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@ARTICLE{Blombach:59150,
      author       = {Blombach, B. and Schreiner, M. E. and Holatko, J. and
                      Bartek, T. and Oldiges, M. and Eikmanns, B. J.},
      title        = {{L}-{V}aline {P}roduction with {P}yruvate {D}ehydrogenase
                      {C}omplex-{D}eficient {C}orynebacterium glutamicum},
      journal      = {Applied and environmental microbiology},
      volume       = {73},
      issn         = {0099-2240},
      address      = {Washington, DC [u.a.]},
      publisher    = {Soc.},
      reportid     = {PreJuSER-59150},
      pages        = {2079 - 2084},
      year         = {2007},
      note         = {Record converted from VDB: 12.11.2012},
      abstract     = {Corynebacterium glutamicum was engineered for the
                      production of L-valine from glucose by deletion of the aceE
                      gene encoding the E1p enzyme of the pyruvate dehydrogenase
                      complex and additional overexpression of the ilvBNCE genes
                      encoding the L-valine biosynthetic enzymes acetohydroxyacid
                      synthase, isomeroreductase, and transaminase B. In the
                      absence of cellular growth, C. glutamicum DeltaaceE showed a
                      relatively high intracellular concentration of pyruvate
                      (25.9 mM) and produced significant amounts of pyruvate,
                      L-alanine, and L-valine from glucose as the sole carbon
                      source. Lactate or acetate was not formed. Plasmid-bound
                      overexpression of ilvBNCE in C. glutamicum DeltaaceE
                      resulted in an approximately 10-fold-lower intracellular
                      pyruvate concentration (2.3 mM) and a shift of the
                      extracellular product pattern from pyruvate and L-alanine
                      towards L-valine. In fed-batch fermentations at high cell
                      densities and an excess of glucose, C. glutamicum
                      DeltaaceE(pJC4ilvBNCE) produced up to 210 mM L-valine with a
                      volumetric productivity of 10.0 mM h(-1) (1.17 g l(-1)
                      h(-1)) and a maximum yield of about 0.6 mol per mol (0.4 g
                      per g) of glucose.},
      keywords     = {Alanine: biosynthesis / Corynebacterium glutamicum:
                      metabolism / Fermentation / Isoleucine: biosynthesis /
                      Lysine: biosynthesis / Pyruvate Dehydrogenase Complex:
                      genetics / Pyruvate Dehydrogenase Complex: physiology /
                      Pyruvic Acid: metabolism / Valine: biosynthesis / Pyruvate
                      Dehydrogenase Complex (NLM Chemicals) / Pyruvic Acid (NLM
                      Chemicals) / Alanine (NLM Chemicals) / Lysine (NLM
                      Chemicals) / Valine (NLM Chemicals) / Isoleucine (NLM
                      Chemicals) / J (WoSType)},
      cin          = {IBT-2},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB56},
      pnm          = {Biotechnologie},
      pid          = {G:(DE-Juel1)FUEK410},
      shelfmark    = {Biotechnology $\&$ Applied Microbiology / Microbiology},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:17293513},
      pmc          = {pmc:PMC1855657},
      UT           = {WOS:000245576300006},
      doi          = {10.1128/AEM.02826-06},
      url          = {https://juser.fz-juelich.de/record/59150},
}