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| Home > Publications database > L-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum > print |
| 001 | 59150 | ||
| 005 | 20190625111501.0 | ||
| 024 | 7 | _ | |2 pmid |a pmid:17293513 |
| 024 | 7 | _ | |2 pmc |a pmc:PMC1855657 |
| 024 | 7 | _ | |2 DOI |a 10.1128/AEM.02826-06 |
| 024 | 7 | _ | |2 WOS |a WOS:000245576300006 |
| 024 | 7 | _ | |a altmetric:21817993 |2 altmetric |
| 037 | _ | _ | |a PreJuSER-59150 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 570 |
| 084 | _ | _ | |2 WoS |a Biotechnology & Applied Microbiology |
| 084 | _ | _ | |2 WoS |a Microbiology |
| 100 | 1 | _ | |a Blombach, B. |b 0 |0 P:(DE-HGF)0 |
| 245 | _ | _ | |a L-Valine Production with Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum |
| 260 | _ | _ | |a Washington, DC [u.a.] |b Soc. |c 2007 |
| 300 | _ | _ | |a 2079 - 2084 |
| 336 | 7 | _ | |a Journal Article |0 PUB:(DE-HGF)16 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a article |2 DRIVER |
| 440 | _ | 0 | |a Applied and Environmental Microbiology |x 0099-2240 |0 8561 |y 7 |v 73 |
| 500 | _ | _ | |a Record converted from VDB: 12.11.2012 |
| 520 | _ | _ | |a Corynebacterium glutamicum was engineered for the production of L-valine from glucose by deletion of the aceE gene encoding the E1p enzyme of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. In the absence of cellular growth, C. glutamicum DeltaaceE showed a relatively high intracellular concentration of pyruvate (25.9 mM) and produced significant amounts of pyruvate, L-alanine, and L-valine from glucose as the sole carbon source. Lactate or acetate was not formed. Plasmid-bound overexpression of ilvBNCE in C. glutamicum DeltaaceE resulted in an approximately 10-fold-lower intracellular pyruvate concentration (2.3 mM) and a shift of the extracellular product pattern from pyruvate and L-alanine towards L-valine. In fed-batch fermentations at high cell densities and an excess of glucose, C. glutamicum DeltaaceE(pJC4ilvBNCE) produced up to 210 mM L-valine with a volumetric productivity of 10.0 mM h(-1) (1.17 g l(-1) h(-1)) and a maximum yield of about 0.6 mol per mol (0.4 g per g) of glucose. |
| 536 | _ | _ | |a Biotechnologie |c PBT |2 G:(DE-HGF) |0 G:(DE-Juel1)FUEK410 |x 0 |
| 588 | _ | _ | |a Dataset connected to Web of Science, Pubmed |
| 650 | _ | 2 | |2 MeSH |a Alanine: biosynthesis |
| 650 | _ | 2 | |2 MeSH |a Corynebacterium glutamicum: metabolism |
| 650 | _ | 2 | |2 MeSH |a Fermentation |
| 650 | _ | 2 | |2 MeSH |a Isoleucine: biosynthesis |
| 650 | _ | 2 | |2 MeSH |a Lysine: biosynthesis |
| 650 | _ | 2 | |2 MeSH |a Pyruvate Dehydrogenase Complex: genetics |
| 650 | _ | 2 | |2 MeSH |a Pyruvate Dehydrogenase Complex: physiology |
| 650 | _ | 2 | |2 MeSH |a Pyruvic Acid: metabolism |
| 650 | _ | 2 | |2 MeSH |a Valine: biosynthesis |
| 650 | _ | 7 | |0 0 |2 NLM Chemicals |a Pyruvate Dehydrogenase Complex |
| 650 | _ | 7 | |0 127-17-3 |2 NLM Chemicals |a Pyruvic Acid |
| 650 | _ | 7 | |0 56-41-7 |2 NLM Chemicals |a Alanine |
| 650 | _ | 7 | |0 56-87-1 |2 NLM Chemicals |a Lysine |
| 650 | _ | 7 | |0 7004-03-7 |2 NLM Chemicals |a Valine |
| 650 | _ | 7 | |0 73-32-5 |2 NLM Chemicals |a Isoleucine |
| 650 | _ | 7 | |a J |2 WoSType |
| 700 | 1 | _ | |a Schreiner, M. E. |b 1 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Holatko, J. |b 2 |0 P:(DE-HGF)0 |
| 700 | 1 | _ | |a Bartek, T. |b 3 |u FZJ |0 P:(DE-Juel1)VDB59554 |
| 700 | 1 | _ | |a Oldiges, M. |b 4 |u FZJ |0 P:(DE-Juel1)129053 |
| 700 | 1 | _ | |a Eikmanns, B. J. |b 5 |0 P:(DE-HGF)0 |
| 773 | _ | _ | |a 10.1128/AEM.02826-06 |g Vol. 73, p. 2079 - 2084 |p 2079 - 2084 |q 73<2079 - 2084 |0 PERI:(DE-600)1478346-0 |t Applied and environmental microbiology |v 73 |y 2007 |x 0099-2240 |
| 856 | 7 | _ | |2 Pubmed Central |u http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1855657 |
| 909 | C | O | |o oai:juser.fz-juelich.de:59150 |p VDB |
| 913 | 1 | _ | |k PBT |v Biotechnologie |l ohne FE |b außerhalb PoF |0 G:(DE-Juel1)FUEK410 |x 0 |
| 914 | 1 | _ | |y 2007 |
| 915 | _ | _ | |0 StatID:(DE-HGF)0010 |a JCR/ISI refereed |
| 920 | 1 | _ | |k IBT-2 |l Biotechnologie 2 |g IBT |z ab 31.10.10 weitergeführt IBG-1 |0 I:(DE-Juel1)VDB56 |x 1 |
| 970 | _ | _ | |a VDB:(DE-Juel1)93111 |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a ConvertedRecord |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a I:(DE-Juel1)IBG-1-20101118 |
| 980 | _ | _ | |a UNRESTRICTED |
| 981 | _ | _ | |a I:(DE-Juel1)IBG-1-20101118 |
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